LOCUS       sequence-165117-       11503 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11503)
  AUTHORS   Alexandrov A, Shu MD, Steitz JA
  TITLE     Fluorescence Amplification Method for Forward Genetic Discovery of 
            Factors in Human mRNA Degradation.
  JOURNAL   Mol Cell. 2017 Jan 5;65(1):191-201. doi: 
            10.1016/j.molcel.2016.11.032. Epub 2016 Dec 22.
  PUBMED    28017590
REFERENCE   2  (bases 1 to 11503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.11.032"; journalName: "Mol Cell"; date: 
            "2017-01-5- 5"; volume: "65"; issue: "1"; pages: "191-201"
COMMENT     Sequence Label: Launchpad (AVA2590)
FEATURES             Location/Qualifiers
     source          1..11503
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     232..252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1873..2106
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2368..2485
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2543..2563)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2561..2858
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2728..2862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2872..2919
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    3033..3114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3070..3089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3124..3143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(3153..3866,3867..3869,3870..3872))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3156..3866,3867..3869,3870..3872))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3198..3219)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3547..3566
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3806..3827
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3908..5092)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3917..4861
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3940..3960)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5394..5893
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5429..5452)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5826..5845
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5914..6513
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5914..5933)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6410..6430
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    6520..6567
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    6856..7080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7213..7232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(8253..8270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8424..9012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8504..8523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9183..9974,9975..10043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9806..9825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10044..10148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10216..10234
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10285..10307)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10393..10531
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10446..10465
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggagtatcta gatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgactga
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgc taatgggagt ttgttttggc
      181 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      241 gcggtaggcg tgtactctag aaggtctata taagcagagc tcgtttagtg aaccgtttat
      301 actacttatc tggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc agaggagatc
      541 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      601 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      661 gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa ggccaggggg
      721 aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag aacgattcgc
      781 agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg gacagctaca
      841 accatccctt cagacaggat cagaagaact tagatcatta tataatacaa tagcagtcct
      901 ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag ataagataga
      961 ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca caggaaacaa
     1021 cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa tggtacatca
     1081 ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga aggctttcag
     1141 cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac aagatttaaa
     1201 taccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa aagagaccat
     1261 caatgaggaa gctgcagaat gggatagatt gcatccagtg catgcagggc ctattgcacc
     1321 aggccagatg agagaaccaa ggggaagtga catagcagga actactagca aattaagaga
     1381 acaatttgga aataataaaa caataatctt taagcaatcc tcaggagggg acccagaaat
     1441 tgtaacgcac agttttaatt gtggagggga atttttctac tgtaattcaa cacaactgtt
     1501 taatagtact tggtttaata gtacttggag tactgaaggg tcaaataaca ctgaaggaag
     1561 tgacacaatc acactcccat gcagaataaa acaatttata aacatgtggc aggaagtagg
     1621 aaaagcaatg tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg
     1681 gctgctatta acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg
     1741 aggaggcgat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1801 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1861 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1921 cgcacggtca atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca
     1981 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     2041 gggcatcaaa cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     2101 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     2161 tgctagttgg agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg
     2221 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2281 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggctt gcggccggaa
     2341 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2401 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2461 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     2521 ggcggccgcg cgaggcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     2581 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     2641 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2701 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     2761 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     2821 tctgagctat tccagaagta gtgaggaggc ttttttggag gctaccatgg agaagttcct
     2881 attccgaagt tcctattctc tagaaagtat aggaacttca agcttggtcg accctcttaa
     2941 gtaccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     3001 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     3061 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3121 gttgtggttt gtccaaactc atcgagctcg acttacttgt acagctcgtc catgccgaga
     3181 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
     3241 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
     3301 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
     3361 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag
     3421 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
     3481 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
     3541 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
     3601 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
     3661 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
     3721 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
     3781 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
     3841 ccggtgaaca gctcctcgcc cttgctcacc atggtggact agttctagag cggaaacgct
     3901 agagttttca cgacacctga aatggaagaa aaaaactttg aaccactgtc tgaggcttga
     3961 gaatgaacca agatccaaac tcaaaaaggg caaattccaa ggagaattac atcaagtgcc
     4021 aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc gcataaaacc
     4081 cctcccccca acctaaagac gacgtactcc aaaagctcga gaactaatcg aggtgcctgg
     4141 acggcgcccg gtactccgtg gagtcacatg aagcgacggc tgaggacgga aaggcccttt
     4201 tcctttgtgt gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc
     4261 tccctgcagc agggccggga agcggccatc tttccgctca cgcaactggt gccgaccggg
     4321 ccagcctgtt gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc
     4381 aggccggcca gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg
     4441 cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac
     4501 cgaaatacca gtgtgcagat cttggcccgc atttacaaga ctatcttgcc agaaaaaaag
     4561 cgtcgcagca ggtcatcaaa aattttaaat ggctagagac ttatcgaaag cagcgagaca
     4621 ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac
     4681 tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc gaactctccc acccacttcc
     4741 aacccgaagc tcgggatcaa gaatcacgta ctgcagccag gggcgtggaa gtaattcaag
     4801 gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac acggcactta
     4861 cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta
     4921 tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc
     4981 agtttacccc gcgccacttt ctctaggcac cggttcaatt gccgacccct ccccccaact
     5041 tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag cctcacgcat
     5101 gctcttctcc acctcagtga tgacgagagc gggcgggtga gggggcggga acgcagcgat
     5161 ctctgggttc tacgttagtg ggagtttaac gacggtccct gggattcccc aaggcagggg
     5221 cgagtccttt tgtatgaatt actctcagct ccggtcgggg cgggttgggg ggggtggtga
     5281 cggggaggcc gcctggaagg gacgtgcaga atcttccctc taccattgct ggcttagctc
     5341 caaagcttat cgatgataag ctgtcaaaca tgagaattgc tagcaattct accgggtagg
     5401 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     5461 gcgctacaca agtggcctct ggcctcgcac acattccaca tccatcggta ggcgccaacc
     5521 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     5581 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     5641 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     5701 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     5761 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     5821 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     5881 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     5941 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6001 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6061 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6121 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6181 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6241 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6301 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6361 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6421 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6481 acctggtgca tgacccgcaa gcccggtgcc tgaggatccg aagttcctat tccgaagttc
     6541 ctattctcta gaaagtatag gaacttcaga tccgcggact agtgaattcc agctgagcgc
     6601 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcagggggga
     6661 tctcgactct agaagcttgg tacctttaag accaatgact tacaaggcag ctgtagatct
     6721 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc agggtctctc
     6781 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6841 cctcataaag ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6901 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6961 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7021 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7081 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     7141 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7201 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctgtg
     7261 cgggagaacg gagttctatt atgactcaaa tcagtctccc caagcattcg gggatcagag
     7321 tttttaagga taacttagtg tgtagggggc cagtgagttg gagatgaaag cgtagggagt
     7381 cgaaggtgtc cttttgcgcc gagtcagttc ctgggtgggg gccacaagat cggatgagcc
     7441 agtttatcaa tccgggggtg ccagctgatc catggagtgc agggtctgca aaatatctca
     7501 agcactgatt gatcttaggt tttacaatag tgatgttacc ccaggaacaa tttggggaag
     7561 gtcagaatct tgtagcctgt agctgcatga ctcctaaacc ataatttctt ttttgttttt
     7621 ttttttttat ttttgagaca gggtctcact ctgtcaccta ggctggagtg cagtggtgca
     7681 atcacagctc actgcagcct caacgtcgta agctcaagcg atcctcccac ctcagcctgc
     7741 ctggtagctg agactacaag cgacgcccca gttaattttt gtatttttgg tagaggcagc
     7801 gttttgccgt gtggccctgg ctggtctcga actcctgggc tcaagtgatc cagcctcagc
     7861 ctcccaaagt gctgggacaa ccggggccag tcactgcacc tggccctaaa ccataatttc
     7921 taatcttttg gctaatttgt tagtcctaca aaggcagtct agtccccagg caaaaagggg
     7981 gtttgtttcg ggaaagggct gttactgtct ttgtttcaaa ctataaacta agttcctcct
     8041 aaacttagtt cggcctacac ccaggaatga acaaggagag cttggaggtt agaagcacga
     8101 tggaattggt taggtcagat ctctttcact gtctgagtta taattttgca atggtggttc
     8161 aaagactgcc cgcttctgac accagtcgct gcattaatga atcggccaac gcgcggggag
     8221 aggcggtttg cgtattggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8281 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8341 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8401 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8461 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8521 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8581 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     8641 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8701 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8761 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8821 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     8881 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8941 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9001 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9061 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9121 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9181 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9241 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9301 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9361 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9421 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9481 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9541 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9601 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9661 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9721 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9781 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9841 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9901 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9961 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10021 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10081 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10141 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    10201 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga actgcctcgc
    10261 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10321 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10381 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt actggcttaa
    10441 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10501 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    10561 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcgcgggg aggcagagat
    10621 tgcagtaagc tgagatcgca gcactgcact ccagcctggg cgacagagta agactctgtc
    10681 tcaaaaataa aataaataaa tcaatcagat attccaatct tttcctttat ttatttattt
    10741 attttctatt ttggaaacac agtccttcct tattccagaa ttacacatat attctatttt
    10801 tctttatatg ctccagtttt ttttagacct tcacctgaaa tgtgtgtata caaaatctag
    10861 gccagtccag cagagcctaa aggtaaaaaa taaaataata aaaaataaat aaaatctagc
    10921 tcactccttc acatcaaaat ggagatacag ctgttagcat taaataccaa ataacccatc
    10981 ttgtcctcaa taattttaag cgcctctctc caccacatct aactcctgtc aaaggcatgt
    11041 gccccttccg ggcgctctgc tgtgctgcca accaactggc atgtggactc tgcagggtcc
    11101 ctaactgcca agccccacag tgtgccctga ggctgcccct tccttctagc ggctgccccc
    11161 actcggcttt gctttcccta gtttcagtta cttgcgttca gccaaggtct gaaactaggt
    11221 gcgcacagag cggtaagact gcgagagaaa gagaccagct ttacaggggg tttatcacag
    11281 tgcaccctga cagtcgtcag cctcacaggg ggtttatcac attgcaccct gacagtcgtc
    11341 agcctcacag ggggtttatc acagtgcacc cttacaatca ttccatttga ttcacaattt
    11401 ttttagtctc tactgtgcct aacttgtaag ttaaatttga tcagaggtgt gttcccagag
    11461 gggaaaacag tatatacagg gttcagtact atcgcatttc agg
//
