LOCUS       sequence-165121-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rhesus AAVS1 safe harbor gene targeting donor expressing CAG-driven 
            copGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     Rhesus AAVS1-CAG-copGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Rhesus AAVS1-CAG-copGFP
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(146..808)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     primer_bind     complement(869..888)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag
       61 tgaattcacg cgtttagcga gatccggtgg agccgggtcc ggcggtgccg tccacggcag
      121 aattggacga ctgagcgcgg gatctggcga aggcgatggg ggtcttgaag gcgtgctggt
      181 actccacgat gcccagctcg gtgttgctgt gcagctcctc cacgcggcgg aaggcgaaca
      241 tggggccccc gttctgcagg atgctggggt ggatggcgct cttgaagtgc atgtggctgt
      301 ccaccacgaa gctgtagtag ccgccgtcgc gcaggctgaa ggtgcgggcg aagctgccca
      361 ccagcacgtt atcgcccatg gggtgcaggt gctccacggt ggcgttgctg cggatgatct
      421 tgtcggtgaa gatcacgctg tcctcgggga agccggtgcc caccaccttg aagtcgccga
      481 tcacgcggcc ggcctcgtag cggtagctga agctcacgtg cagcacgccg ccgtcctcgt
      541 acttctcgat gcgggtgttg gtgtagccgc cgttgttgat ggcgtgcagg aaggggttct
      601 cgtagccgct ggggtaggtg ccgaagtggt agaagccgta gcccatcacg tggctcagca
      661 ggtaggggct gaaggtcagg gcgcctttgg tgctcttcat cttgttggtc atgcggccct
      721 gcttgggggt gccctctccg ccgcccacca gctcgaactc cacgccgttc agggtgccgg
      781 tgatgcggca ctcgatctcc atggcgggca ggccgctctc gtcgctctcc atggttgtac
      841 acaattcttt gccaaaatga tgagacagca caataaccag cacgttgccc aggagctgta
      901 ggaaaa
//
