LOCUS       sequence-165125-        5065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' entry vector containing the zebrafish krt5 promoter for 
            epidermis-specific expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Akerberg AA, Stewart S, Stankunas K
  TITLE     Spatial and temporal control of transgene expression in zebrafish.
  JOURNAL   PLoS One. 2014 Mar 18;9(3):e92217. doi: 
            10.1371/journal.pone.0092217. eCollection 2014.
  PUBMED    24643048
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-18"; volume: "9(3):e92217. doi"; pages: " 
            10.1371/journal.pone.0092217. eCollection 2014"
COMMENT     Sequence Label: p5E-krt5
FEATURES             Location/Qualifiers
     source          1..5065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3039..3055)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3039..3055)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3092..3110)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3111..3234
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3307..3325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3330..3346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3330..3346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3448..3467)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3459..4268
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3526..3545)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4415..5003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4904..4923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgggccccc cctcgaggtt cgaacagtgt attgtagttc ctcaaaatag caacgcgcca
      781 gcaatgcgcc tcaacacgcc tcctttttta gaccaaaacg tctattggca cacatactgt
      841 atgagcgcta atgcatttgc tatttaaaca gcgtggtgca aaacgtcaaa atgactgttg
      901 cactgagcta aaactacaat tgcgtcgcgc cttgcgccgg gtgtatgata gggccccaag
      961 agtttattta cttatttaag tgccttggtc cccaatactg aatttactag atcaaaatta
     1021 caatacaacc tttatacttc caaaaacatt atatgtaaat atagttcaaa atataattta
     1081 ttcttatgat ggcaacgcta aatttttagc attgtagttt taaatgtcat gatactgtaa
     1141 aagtcattta aatatggtgg tctgatgctc aaaaatccct ttttattgta tgagttgtgc
     1201 tacttcaaac tgtatttttt atgtaaacgc tcactaaaaa tgtttgactg tcaggaacat
     1261 taaaaatcga cagtaaaagt cattaaaaat gttattaaac atttcagttc taataactac
     1321 tgtttgttaa caatctattc atagatattt taactgtgca gcaaatcaac agtgcctatt
     1381 tttattttat atttgatata ataatagcct aataacagat ataataaaaa cctggatgaa
     1441 cagaagagac aaaatgtctt attgtctcca aaactttaac agatggttct gcaagggaaa
     1501 tgtttacaac catgaaagca tttcaaatat ttaatgagtt tgatcattag caaagcaaac
     1561 acgcaaggta aatttcaaac cagtttcacg tgatcaacct gcacatgtta cacttacctg
     1621 tttttttaat cacatatatg gtttctgggt cttcaagacc tcttttatct cacaagctaa
     1681 tctacatttt gtaacttgta ctactcgatt ggttgcattg catgaattaa ggggaaatac
     1741 ttgggtggaa ctcatcaacc ccaacatctg atagtgtcac tgttttgacc aacggattaa
     1801 caatgggcta aacccgttta agaaaaaaaa aactgcacag atggccaaaa taccacataa
     1861 gcaatgtttg ttaaaagtgc gacccctcct aaatttttac agagaaatat gccttatttt
     1921 ccttttgctt ccctgctaat atgctaatgc tctaaagtaa acaaaccaga cagaatctct
     1981 tcctgctgta cttggtctga tgaatattta caaggctgaa agcagactca gatctgtcca
     2041 ctttagaagc aagactccag ttcgaaatca tttaaacatg atacgggtga tagtgacatg
     2101 ctaacaaaag cagcacgcgt tgctaaataa tgccacctaa ggacaggtac aaaaataacc
     2161 acagtgctct tgcagccatt gcacactgct gtgagctctg acactccccc ttataaaatt
     2221 ccaatctttc acacgataat gaaaaactat gctgtacaca ttcacgtttg cacagtgtgt
     2281 tttaataaga taataaaaaa tctactttgc atgcaactgc gtcagccctc tgggaaccac
     2341 agaacaaggt ggtgaagcag tgtccgtgac agtgccattg aagtgagaga acagaagctt
     2401 gattgtttga ctggaaagag aagcctgtgg gtcactatgg cttccaggat gagcgccaag
     2461 tctcaacatt caagcccttc atcaaatatt cccctattta atactcctgc attgcactta
     2521 aagcaaactg agggccccat acctgcatga atcataaaat attcatggag ttgtatggtt
     2581 tgcatttgga gaatgccatc tggttatgta aataagaggg gctttaaatt acagagaact
     2641 tgtatattct gagtcgtagt acatacaagg gtgtgtggag tttaaccttg tttttctact
     2701 ctgtttctct cttaatagct ctgttttatt acgtgagtat gcatgtgcat ctaaggcagg
     2761 gtgtggcctg gcctccaatg ctgaacatac tcccatacca gcccagcaag atgggcgtgt
     2821 gtatcacttg gacagggaaa aaggcaggac agaagcctga ggcagaaagg ggattggcca
     2881 ggtgagcccc tcccatgagg taagaggtat aaaagccact gcactgcttg aagagggcac
     2941 tcagagggat tttactttcc tcttagtgag ctctcctgct ccagatctct gtctcatcct
     3001 gctctttttc tgatcctcac cccctcgtag acgacaggga tccactagtt ctagagcggc
     3061 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt caagtttgta
     3121 caaaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     3181 gcataaaaaa cagactacat aatactgtaa aacacaacat atgcagtcac tatgaaccaa
     3241 ctacttagat ggtattagtg acctgtagaa ttcgagctct agagctgcag ggcggccgcg
     3301 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     3361 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     3421 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     3481 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     3541 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
     3601 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     3661 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     3721 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     3781 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     3841 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     3901 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     3961 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     4021 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     4081 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     4141 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     4201 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     4261 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     4321 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     4381 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4441 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4501 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4561 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4621 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4681 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4741 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4801 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4861 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4921 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4981 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5041 tttgctggcc ttttgctcac atgtt
//
