LOCUS       sequence-16514-e        1174 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Shorter J, Lindquist S
  TITLE     Hsp104 catalyzes formation and elimination of self-replicating Sup35
            prion conformers.
  JOURNAL   Science 2004 Jun 18;304(5678):1793-7. Epub 2004 May 20.
  PUBMED    15155912
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2004-06-18"; volume: "304(5678)"; pages: "1793-7. Epub 2004 
            May 20"
COMMENT     Sequence Label: 6975 pPROEX-HTb-SIS1
FEATURES             Location/Qualifiers
     source          1..1174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgtnnccatc accatcacca tcacgattac gatatcccaa cgaccgaaaa cctgtatttt
       61 cagggcgcca tgggatccat ggtcaaggag acaaaacttt atgatttact tggagtatct
      121 ccaagtgcta atgagcaaga actgaaaaag ggttatagaa aagcagctct aaaatatcat
      181 ccagataagc caacaggtga cacagaaaag tttaaggaga tatcagaggc ctttgaaatt
      241 ttaaatgatc ctcaaaaaag ggaaatatat gatcaatacg gtctcgaggc tgctagatct
      301 ggtggtccaa gctttggtcc tggtggtcct ggcggtgctg gaggtgctgg aggcttccct
      361 ggcggtgcgg gcggattctc cggaggacat gcgttcagta atgaggatgc tttcaatatt
      421 ttttcacaat tctttggcgg cagttcccca ttcggtggtg ctgatgacag tggcttcagt
      481 ttctctagtt atccatctgg cggcggtgct ggtatgggag gtatgcctgg aggaatggga
      541 ggaatgcatg gcggcatggg aggtatgcct ggcggcttta gatcagcatc aagctctccc
      601 acgtatccag aggaagaaac agttcaagtt aatttaccag ttagtctaga agatttgttt
      661 gttggtaaaa agaagtcatt taaaattgga agaaagggcc cacatggggc ctctgaaaag
      721 acacaaattg acattcaatt aaaaccgggt tggaaagctg gtaccaaaat aacatacaag
      781 aaccagggtg attacaatcc tcaaacgggc cgtagaaaga ctttgcagtt tgtcatccag
      841 gaaaagagcc atccaaactt taaaagagac ggtgatgacc taatttacac tctgccacta
      901 tctttcaagg aatcattgnt taggttttca aaactatccn aacaattgat ggcanaacct
      961 tacctttgtc gagagtacag cctgtccaac cctcacaaac ttctacttat cntggtcaag
     1021 gtatgcccac tccaagaacc catctcaaaa agtaatttga tngtaaatat aaagtggact
     1081 atcccatatc cctaacgacc cccaaacngg ctaaaatgaa aattttaana gnccacaacc
     1141 ccttattccc gncnctttca atntaaancc tgca
//
