LOCUS       sequence-165146-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rhesus AAVS1 safe harbor gene targeting donor expressing CAG-driven 
            copGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     Rhesus AAVS1-CAG-copGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Rhesus AAVS1-CAG-copGFP
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(719..752)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    753..778
                     /label=SA
                     /note="splice acceptor site"
     CDS             802..855
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(859..878)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 accatgatta cgccgccacc tccttcaggt tccagcttcc tccaatccct cttccccttg
       61 ctctgtgctg tgcgttgctg cccaaggatg ctctttccag agcacttcct tctcggcgct
      121 gcaccacgtg gtgtcctcgg agcggatcct gcccttgtct gggtcctctc cgggcatctc
      181 tgctccctac cccaaccccc atgccatctt cactcgctgg gttccctttt ccctctcctt
      241 ctggggcctg tgccatctct cgtttcttag gatggtcttc tccgtgggat gcctctccct
      301 tgcgtcccca cctccccttc tcgtaggcct gcgtcatcac cgtttttccc gacaacccca
      361 aagtccctgt ctccctggct ttagccacct ctccgccctc ttgctttctt tgcctggaca
      421 ccccctcctc ctgtggattt gggtcacctc tcacttcttt aattcgggca gctcccatac
      481 cccctttacc tctctagtct gtgctcgctc ttccggcccc tgtcacggca tcttccaggg
      541 gtcccagagc tcggctggtc ctcttcctcc aagccaggcc cccatggcca cttcaggaca
      601 agatcttgtg tcccagagcc gggaccacct tatattccca ggaccgatta atatggctct
      661 ggttctgggt acttttatct gtaccctcca ccccaccgtg gggccactag ggacaggcat
      721 aacttcgtat agcatacatt atacgaagtt atctgacctc ttctcttcct cccacagggc
      781 ctcgagagat ctggcagcgg agagggcaga ggaagtcttc taacatgcgg tgacgtggag
      841 gagaatcccg gccctaggat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
      901 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
//
