LOCUS       sequence-165161-        5331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CLOCK , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CLOCK_WT
FEATURES             Location/Qualifiers
     source          1..5331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     763..780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(939..966)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1058..1144)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1193..1215
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1207..1224
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1208..1224
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1240..1339
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3881..3980)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3997..4016)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3998..4016)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4021..4037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4021..4037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4468..5259
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      721 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      781 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      841 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      901 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
      961 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     1021 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     1081 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     1141 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     1201 cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga
     1261 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
     1321 ttgtacaaaa aagttggcac catgttgttt accgtaagct gtagtaaaat gagctcgatt
     1381 gttgacagag atgacagtag tatttttgat gggttggtgg aagaagatga caaggacaaa
     1441 gcgaaaagag tatctagaaa caaatctgaa aagaaacgta gagatcaatt taatgttctc
     1501 attaaagaac tgggatccat gcttcctggt aatgctagaa agatggacaa atctactgtt
     1561 ctgcagaaaa gcattgattt tttacgaaaa cataaagaaa tcactgcaca gtcagatgct
     1621 agtgaaattc gacaggactg gaaacctaca ttccttagta atgaagagtt tacacaatta
     1681 atgttagagg ctcttgatgg ttttttttta gcaatcatga cagatggaag cataatatat
     1741 gtgtctgaga gtgtaacttc attacttgaa catttaccat ctgatcttgt ggatcaaagt
     1801 atatttaatt ttatcccaga aggggaacat tcagaggttt ataaaatact ctctactcat
     1861 ctgctggaaa gtgattcatt aaccccagaa tatttaaaat caaaaaatca gttagaattc
     1921 tgttgtcaca tgctgcgagg aacaatagac ccaaaggagc catctaccta tgaatatgta
     1981 aaatttatag gaaatttcaa atctttaaac agtgtatcct cttcagcaca caatggtttt
     2041 gaaggaacta tacaacgcac acataggcca tcttatgaag atagagtttg ttttgtagct
     2101 actgtcaggt tagctacacc tcagttcatc aaggaaatgt gcactgttga agaacccaat
     2161 gaagagttta catctagaca tagtttagaa tggaagtttc tgtttctaga tcacagggca
     2221 ccacccataa tagggtattt gccatttgaa gttctgggaa catcaggcta tgattactat
     2281 catgtggatg acctagaaaa tttggcaaaa tgtcatgagc acttaatgca atatgggaaa
     2341 ggcaaatcat gttattatag gttcctgact aaggggcaac agtggatttg gcttcagact
     2401 cattattata tcacttacca tcagtggaat tcaaggccag agtttattgt ttgtactcac
     2461 actgtagtaa gttatgcaga agttagggct gaaagacgac gagaacttgg cattgaagag
     2521 tctcttcctg agacagctgc tgacaaaagc caagattctg ggtcagataa tcgtataaac
     2581 acagtcagtc tcaaggaagc attggaaagg tttgatcaca gcccaacccc ttctgcctct
     2641 tctcggagtt caagaaaatc atctcacacg gccgtctcag acccttcctc aacaccaacc
     2701 aagatcccga cggatacgag cactccaccc aggcagcatt taccagctca tgagaagatg
     2761 gtgcaaagaa ggtcatcatt tagtagtcag tccataaatt cccagtctgt tggttcatca
     2821 ttaacacagc cagtgatgtc tcaagctaca aatttaccaa ttccacaagg catgtcccag
     2881 tttcagtttt cagctcaatt aggagccatg caacatctga aagaccaatt ggaacaacgg
     2941 acacgcatga tagaagcaaa tattcatcgg caacaagaag aactaagaaa aattcaagaa
     3001 caacttcaga tggtccatgg tcaggggctg cagatgtttt tgcaacaatc aaatcctggg
     3061 ttgaattttg gttccgttca actttcttct ggaaattcat ctaacatcca gcaacttgca
     3121 cctataaata tgcaaggcca agttgttcct actaaccaga ttcaaagtgg aatgaatact
     3181 ggacacattg gcacaactca gcacatgata caacaacaga ctttacagag tacatcaact
     3241 cagagtcaac aaaatgtact gagtgggcac agtcagcaaa catctctacc cagtcagaca
     3301 cagagcactc ttacagcccc actgtataac actatggtga tttctcagcc tgcagccgga
     3361 agcatggtcc agattccatc tagtatgcca caaaacagca cccagagtgc tgcagtaact
     3421 acattcactc aggacaggca gataagattt tctcaaggtc aacaacttgt gaccaaatta
     3481 gtgactgctc ctgtagcttg tggggcagtc atggtaccta gtactatgct tatgggccag
     3541 gtggtgactg catatcctac ttttgctaca caacagcaac agtcacagac attgtcagta
     3601 acgcagcagc agcagcagca gagctcccag gagcagcagc tcacttcagt tcagcaacca
     3661 tctcaggctc agctgaccca gccaccgcaa caatttttac agacttctag gttgctccat
     3721 gggaatccct caactcaact cattctctct gctgcatttc ctctacaaca gagcaccttc
     3781 cctcagtcac atcaccagca acatcagtct cagcaacagc agcaactcag ccggcacagg
     3841 actgacagct tgcccgaccc ttccaaggtt caaccacagt tgccaacttt cttgtacaaa
     3901 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     3961 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     4021 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     4081 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     4141 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     4201 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     4261 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     4321 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     4381 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     4441 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     4501 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     4561 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     4621 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     4681 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     4741 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     4801 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     4861 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     4921 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     4981 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     5041 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     5101 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     5161 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     5221 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     5281 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt a
//
