LOCUS       sequence-165163-        4686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing IKBKE, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IKBKE_WT
FEATURES             Location/Qualifiers
     source          1..4686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      18..606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     507..526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     760..777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(936..963)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1055..1141)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1190..1212
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1204..1221
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1205..1221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1237..1336
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3233..3332)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3349..3368)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3350..3368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3373..3389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3373..3389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3820..4611
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
       61 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      661 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      721 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      781 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      841 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
      901 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
      961 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     1021 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     1081 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     1141 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     1201 cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg
     1261 atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg
     1321 tacaaaaaag ttggcaccat ggagtactgc tccagtggga gcctgctgag tgtgctggag
     1381 agccctgaga atgcctttgg gctgcctgag gatgagttcc tggtggtgct gcgctgtgtg
     1441 gtggccggca tgaaccacct gcgggagaac ggcattgtgc atcgcgacat caagccgggg
     1501 aacatcatgc gcctcgtagg ggaggagggg cagagcatct acaagctgac agacttcggc
     1561 gctgcccggg agctggatga tgatgagaag ttcgtctcgg tctatgggac tgaggagtac
     1621 ctgcatcccg acatgtatga gcgggcggtg cttcgaaagc cccagcaaaa agcgttcggg
     1681 gtgactgtgg atctctggag cattggagtg accttgtacc atgcagccac tggcagcctg
     1741 cccttcatcc cctttggtgg gccacggcgg aacaaggaga tcatgtaccg gatcaccacg
     1801 gagaagccgg ctggggccat tgcaggtgcc cagaggcggg agaacgggcc cctggagtgg
     1861 agctacaccc tccccatcac ctgccagctg tcactggggc tgcagagcca gctggtgccc
     1921 atcctggcca acatcctgga ggtggagcag gccaagtgct ggggcttcga ccagttcttt
     1981 gcggagacca gtgacatcct gcagcgagtt gtcgtccatg tcttctccct gtcccaggca
     2041 gtcctgcacc acatctatat ccatgcccac aacacgatag ccattttcca ggaggccgtg
     2101 cacaagcaga ccagtgtggc cccccgacac caggagtacc tctttgaggg tcacctctgt
     2161 gtcctcgagc ccagcgtctc agcacagcac atcgcccaca cgacggcaag cagccccctg
     2221 accctcttca gcacagccat ccctaagggg ctggccttca gggaccctgc tctggacgtc
     2281 cccaagttcg tccccaaagt ggacctgcag gcggattaca acactgccaa gggcgtgttg
     2341 ggcgccggct accaggccct gcggctggca cgggccctgc tggatgggca ggagctaatg
     2401 tttcgggggc tgcactgggt catggaggtg ctccaggcca catgcagacg gactctggaa
     2461 gtggcaagga catccctcct ctacctcagc agcagcctgg gaactgagag gttcagcagc
     2521 gtggctggaa cgcctgagat ccaggaactg aaggcggctg cagaactgag gtccaggctg
     2581 cggactctag cggaggtcct ctccagatgc tcccaaaata tcacggagac ccaggagagc
     2641 ctgagcagcc tgaaccggga gctggtgaag agccgggatc aggtacatga ggacagaagc
     2701 atccagcaga ttcagtgctg tttggacaag atgaacttca tctacaaaca gttcaagaag
     2761 tctaggatga ggccagggct tggctacaac gaggagcaga ttcacaagct ggataaggtg
     2821 aatttcagtc atttagccaa aagactcctg caggtgttcc aggaggagtg cgtgcagaag
     2881 tatcaagcgt ccttagtcac acacggcaag aggatgaggg tggtgcacga gaccaggaac
     2941 cacctgcgcc tggttggctg ttctgtggct gcctgtaaca cagaagccca gggggtccag
     3001 gagagtctca gcaagctcct ggaagagcta tctcaccagc tccttcagga ccgagcaaag
     3061 ggggctcagg cctcgccgcc tcccatagct ccttacccca gccctacacg aaaggacctg
     3121 cttctccaca tgcaagagct ctgcgagggg atgaagctgc tggcatctga cctcctggac
     3181 aacaaccgca tcatcgaacg gctaaataga gtcccagcac ctcctgatgt cttgccaact
     3241 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     3301 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     3361 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     3421 tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga
     3481 aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg
     3541 aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     3601 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     3661 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     3721 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     3781 acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc
     3841 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     3901 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     3961 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     4021 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     4081 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     4141 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     4201 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     4261 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     4321 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     4381 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     4441 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     4501 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     4561 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc
     4621 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     4681 gtagaa
//
