LOCUS       sequence-165164-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-VASP-5
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             51..746
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagcg
       61 agctgattaa ggagaacatg cccatgaagc tgtacatgga gggcaccgtg aacaaccacc
      121 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
      181 tcaaggtcgt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
      241 tgtacggcag cagaaccttc atcaagcacc ctccgggcat ccccgacttc tttaagcagt
      301 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
      361 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aaggttagag
      421 gggtgaactt cccagccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
      481 ccaccgagac gatgtacccc gctgacggcg gcctggaagg cgcatgtgac atggccctga
      541 agctcgtggg cgggggccac ctgatctgca accttgagac cacatacaga tccaagaaac
      601 ccgctacgaa cctcaagatg cccggcgtct acaacgtgga ccacagactg gaaagaatca
      661 aggaggccga cgatgagacc tacgtcgagc agcacgaggt ggctgtggcc agatactcta
      721 ctggtggcgc tggtgatgga ggtaaaggtg gaggaggttc cggactcaga tctatgagcg
      781 agacggtcat ctgttccagc cgggctactg tgatgcttta tgatgacagc aacaagcgat
      841 ggctccctgc tggcac
//
