LOCUS       sequence-165171-        3588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DKK1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DKK1_WT
FEATURES             Location/Qualifiers
     source          1..3588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      58..646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     547..566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     800..817
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(976..1003)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1095..1181)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1230..1252
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1244..1261
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1245..1261
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1277..1376
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2175..2274)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2291..2310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2292..2310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2315..2331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2315..2331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2762..3553
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
       61 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
      121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
      181 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
      241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
      301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
      361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
      421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
      481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
      541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
      601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
      661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
      721 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
      781 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
      841 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      901 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
      961 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
     1021 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
     1081 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     1141 cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac
     1201 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
     1261 tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca
     1321 acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcatgat
     1381 ggctctgggc gcagcgggag ctacccgggt ctttgtcgcg atggtagcgg cggctctcgg
     1441 cggccaccct ctgctgggag tgagcgccac cttgaactcg gttctcaatt ccaacgctat
     1501 caagaacctg cccccaccgc tgggcggcgc tgcggggcac ccaggctctg cagtcagcgc
     1561 cgcgccggga atcctgtacc cgggcgggaa taagtaccag accattgaca actaccagcc
     1621 gtacccgtgc gcagaggacg aggagtgcgg cactgatgag tactgcgcta gtcccacccg
     1681 cggaggggac gcaggcgtgc aaatctgtct cgcctgcagg aagcgccgaa aacgctgcat
     1741 gcgtcacgct atgtgctgcc ccgggaatta ctgcaaaaat ggaatatgtg tgtcttctga
     1801 tcaaaatcat ttccgaggag aaattgagga aaccatcact gaaagctttg gtaatgatca
     1861 tagcaccttg gatgggtatt ccagaagaac caccttgtct tcaaaaatgt atcacaccaa
     1921 aggacaagaa ggttctgttt gtctccggtc atcagactgt gcctcaggat tgtgttgtgc
     1981 tagacacttc tggtccaaga tctgtaaacc tgtcctgaaa gaaggtcaag tgtgtaccaa
     2041 gcataggaga aaaggctctc atggactaga aatattccag cgttgttact gtggagaagg
     2101 tctgtcttgc cggatacaga aagatcacca tcaagccagt aattcttcta ggcttcacac
     2161 ttgtcagaga cactacccaa ctttcttgta caaagttggc attataagaa agcattgctt
     2221 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2281 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2341 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     2401 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
     2461 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     2521 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     2581 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     2641 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     2701 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     2761 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2821 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2881 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2941 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     3001 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     3061 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     3121 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     3181 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     3241 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     3301 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     3361 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     3421 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     3481 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     3541 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgt
//
