LOCUS       sequence-165175-        4278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SGK3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SGK3_WT
FEATURES             Location/Qualifiers
     source          1..4278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     525..544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     778..795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(954..981)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1073..1159)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1208..1230
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1222..1239
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1223..1239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1255..1354
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2843..2942)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2959..2978)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2960..2978)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2983..2999)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2983..2999)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3430..4221
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      181 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      421 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      781 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      841 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      901 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
      961 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     1021 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     1081 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     1141 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     1201 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
     1261 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
     1321 tttataatgc caactttgta caaaaaagtt ggcatgcaaa gagatcacac catggactac
     1381 aaggaaagct gcccaagtgt aagcattccc agctccgatg aacacagaga gaaaaagaag
     1441 aggtttactg tttataaagt tctggtttca gtgggaagaa gtgaatggtt tgtcttcagg
     1501 agatatgcag agtttgataa actttataac actttaaaaa aacagtttcc tgctatggcc
     1561 ctgaagattc ctgccaagag aatatttggt gataattttg atccagattt tattaaacaa
     1621 agacgagcag gactaaacga attcattcag aacctagtta ggtatccaga actttataac
     1681 catccagatg tcagagcatt ccttcaaatg gacagtccaa aacaccagtc agatccatct
     1741 gaagatgagg atgaaagaag ttctcagaag ctacactcta cctcacagaa catcaacctg
     1801 ggaccgtctg gaaatcctca tgccaaacca actgactttg atttcttaaa agttattgga
     1861 aaaggcagct ttggcaaggt tcttcttgca aaacggaaac tggatggaaa attttatgct
     1921 gtcaaagtgt tacagaaaaa aatagttctc aacagaaaag agcaaaaaca tattatggct
     1981 gaacgtaatg tgctcttgaa aaatgtgaaa catccgtttt tggttggatt gcattattcc
     2041 ttccaaacaa ctgaaaagct ttattttgtt ctggattttg ttaatggagg ggagcttttt
     2101 ttccacttac aaagagaacg gtcctttcct gagcacagag ctaggtttta cgctgctgaa
     2161 attgctagtg cattgggtta cttacattcc atcaaaatag tatacagaga cttgaaacca
     2221 gaaaatattc ttttggattc agtaggacat gttgtcttaa cagattttgg gctttgtaaa
     2281 gaaggaattg ctatttctga caccactacc acattttgtg ggacaccaga gtatcttgca
     2341 cctgaagtaa ttagaaaaca gccctatgac aatactgtag attggtggtg ccttggggct
     2401 gttctgtatg aaatgctgta tggattgcct cctttttatt gccgagatgt tgctgaaatg
     2461 tatgacaata tccttcacaa acccctaagt ttgaggccag gagtgagtct tacagcctgg
     2521 tccattctgg aagaactcct agaaaaagac aggcaaaatc gacttggtgc caaggaagac
     2581 tttcttgaaa ttcagaatca tccttttttt gaatcactca gctgggctga ccttgtacaa
     2641 aagaagattc caccaccatt taatcctaat gtggctggac cagatgatat cagaaacttt
     2701 gacacagcat ttacagaaga aacagttcca tattctgtgt gtgtatcttc tgactattct
     2761 atagtgaatg ccagtgtatt ggaggcagat gatgcattcg ttggtttctc ttatgcacct
     2821 ccttcagaag acttattttt gtgcccaact ttcttgtaca aagttggcat tataagaaag
     2881 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     2941 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     3001 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     3061 tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt
     3121 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     3181 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     3241 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     3301 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     3361 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     3421 ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     3481 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     3541 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     3601 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     3661 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     3721 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     3781 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     3841 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     3901 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     3961 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     4021 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     4081 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     4141 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     4201 accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt
     4261 tacgcgtcgt tccactga
//
