LOCUS       sequence-165178-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: mMaroon-PAK-mMaroon
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             53..784
                     /codon_start=1
                     /product="bright, rapidly maturing, monomeric far-red 
                     fluorescent protein (Bajar et al., 2016)"
                     /label=mMaroon1
                     /translation="MVSKGEELIKENMHTKLYLTGTVNNHYFECTAEGEGKPYEGTQTN
                     RIKVVRGGPLPFAFDILAPCFMYGSKTFINHPPDIPDYFKQSFPEGFTWERTTVYEDGG
                     TLTATQDTSLQDGCLIYNVQVRGENFPSNGPVMQKKTLGWEASTETLYPADGSLEGRLD
                     WALKLVGGGHLHCRLETTYRSKKPAKNLKMPGVYFIDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag
       61 caagggcgag gagctgatca aggagaacat gcacaccaag ctgtacctga caggcaccgt
      121 gaacaaccac tacttcgaat gcaccgccga aggggagggc aagccctacg agggcaccca
      181 gaccaacagg atcaaggtgg tgcgcggagg ccccctgccg ttcgcattcg acatcctggc
      241 cccctgcttt atgtacggga gcaagacctt cattaaccat ccacctgaca ttcccgatta
      301 ttttaagcag tccttccctg agggcttcac atgggagaga accaccgttt acgaagacgg
      361 gggcacactc actgctacac aggacaccag cctccaggac ggctgcctga tctacaacgt
      421 ccaagtcaga ggggagaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg
      481 ctgggaggcc agtaccgaga ccctgtaccc cgctgacggc agcctggaag gcagactgga
      541 ctgggccctg aagctcgtgg gcgggggcca cctgcactgc agactggaaa ccacatacag
      601 atccaagaaa cccgcaaaga acctcaagat gcccggcgtc tattttattg acaggagact
      661 ggaaagaatc aaggaggccg acaatgagac ctacgtcgag cagcacgagg tggctgtggc
      721 cagatactgc gacctcccta gcaaactggg gcacaaactt aatggcatgg acgagctgta
      781 caagtccgga ctcagatctc gaggaatgaa caaa
//
