LOCUS       sequence-165181-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CyRFP1 fluorescent protein in an empty backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: CyRFP1-C1
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..755
                     /codon_start=1
                     /product="cyan-excitable red fluorescent protein (Laviv et 
                     al., 2016)"
                     /label=CyRFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
     misc_feature    756..821
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gcaagggcga ggagctgatc aaggagaaca tgagaagcaa gctgtacctg gaaggcagcg
      121 tgaacggcca ccagttcaag tgcacccacg aaggggaggg caagccctac gagggcaagc
      181 agaccaacag gatcaaggtg gtggagggag gccccctgcc gttcgcattc gacatcctgg
      241 ccaccatgtt tatgtacggg agcaaggtgt tcatcaagta ccccgccgac ctccccgatt
      301 attttaagca gtccttccct gagggcttca catgggagag agtcatggtg ttcgaagacg
      361 ggggcgtgct gaccgccacc caggacacca gcctccagga cggcgagctc atctacaacg
      421 tcaagctcag aggggtgaac ttcccagcca acggccccgt gatgcagaag aaaacactgg
      481 gctgggagcc cagcaccgag accatgtacc ccgctgacgg cggcctggaa ggcagatgcg
      541 acaaggccct gaagctcgtg ggcgggggcc acctgcacgt caacttcaag accacataca
      601 agtccaagaa acccgtgaag atgcccggcg tccactacgt ggaccgcaga ctggaaagaa
      661 tcaaggaggc cgacaacgag acctacgtcg agcagtacga gcacgctgtg gccagatact
      721 ccaacctggg cggaggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag
      781 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
      841 tcataatcag ccataccaca tttgtagagg tt
//
