LOCUS       sequence-165183-        3486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DDIT4 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DDIT4_WT
FEATURES             Location/Qualifiers
     source          1..3486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      32..620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     521..540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     774..791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(950..977)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1069..1155)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1204..1226
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1218..1235
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1219..1235
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1251..1350
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2047..2146)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2163..2182)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2164..2182)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2187..2203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2187..2203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2634..3425
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
       61 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      841 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      901 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
      961 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     1021 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     1081 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     1141 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt
     1201 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga
     1261 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
     1321 taatgccaac tttgtacaaa aaagttggca tgcctagcct ttgggaccgc ttctcgtcgt
     1381 cgtccacctc ctcttcgccc tcgtccttgc cccgaactcc caccccagat cggccgccgc
     1441 gctcagcctg ggggtcggcg acccgggagg aggggtttga ccgctccacg agcctggaga
     1501 gctcggactg cgagtccctg gacagcagca acagtggctt cgggccggag gaagacacgg
     1561 cttacctgga tggggtgtcg ttgcccgact tcgagctgct cagtgaccct gaggatgaac
     1621 acttgtgtgc caacctgatg cagctgctgc aggagagcct ggcccaggcg cggctgggct
     1681 ctcgacgccc tgcgcgcctg ctgatgccta gccagttggt aagccaggtg ggcaaagaac
     1741 tactgcgcct ggcctacagc gagccgtgcg gcctgcgggg ggcgctgctg gacgtctgcg
     1801 tggagcaggg caagagctgc cacagcgtgg gccagctggc actcgacccc agcctggtgc
     1861 ccaccttcca gctgaccctc gtgctgcgcc tggactcacg actctggccc aagatccagg
     1921 ggctgtttag ctccgccaac tctcccttcc tccctggctt cagccagtcc ctgacgctga
     1981 gcactggctt ccgagtcatc aagaagaagc tgtacagctc ggaacagctg ctcattgagg
     2041 agtgttgccc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     2101 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     2161 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2221 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     2281 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2341 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2401 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2461 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2521 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2581 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2641 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2701 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2761 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2821 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2881 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2941 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     3001 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     3061 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3121 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3181 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3241 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3301 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3361 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3421 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3481 gagcgt
//
