LOCUS       sequence-165186-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Schirmer EC, Homann OR, Kowal AS, Lindquist S
  TITLE     Dominant gain-of-function mutations in Hsp104p reveal crucial roles 
            for the middle region.
  JOURNAL   Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20.
  PUBMED    14978213
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2004-05"; volume: "15(5)"; pages: "2061-72. Epub 2004 
            Feb 20"
COMMENT     Sequence Label: CDC12(WT)
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(23..41)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    82..585
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        622..726
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(945..964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
       61 ataataatgg tttcttagac ggatcgcttg cctgtaactt acacgcgcct cgtatctttt
      121 aatgatggaa taatttggga atttactctg tgtttattta tttttatgtt ttgtatttgg
      181 attttagaaa gtaaataaag aaggtagaag agttacggaa tgaagaaaaa aaaataaaca
      241 aaggtttaaa aaatttcaac aaaaagcgta ctttacatat atatttatta gacaagaaaa
      301 gcagattaaa tagatataca ttcgattaac gataagtaaa atgtaaaatc acaggatttt
      361 cgtgtgtggt cttctacaca gacaagatga aacaattcgg cattaatacc tgagagcagg
      421 aagagcaaga taaaaggtag tatttgttgg cgatccccct agagtctttt acatcttcgg
      481 aaaacaaaaa ctattttttc tttaatttct ttttttactt tctattttta atttatatat
      541 ttatattaaa aaatttaaat tataattatt tttatagcac gtgatgaaaa ggacccaggt
      601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      961 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1021 gacttggttg agtactcacc a
//
