LOCUS       sequence-165194-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express human caspase 8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Oberst A, Pop C, Tremblay AG, Blais V, Denault JB, Salvesen GS, 
            Green DR
  TITLE     Inducible dimerization and inducible cleavage reveal a requirement 
            for both processes in caspase-8 activation.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16632-42. doi: 
            10.1074/jbc.M109.095083. Epub 2010 Mar 22.
  PUBMED    20308068
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.095083"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16632-42"
COMMENT     Sequence Label: Fv-hCaspase 8 (C/A)-2A-GFP
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(8..721,722..724,725..727))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(11..721,722..724,725..727))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(53..74)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     402..421
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(737..790)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tttaaactta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
       61 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
      121 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      181 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      241 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      301 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      361 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      421 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      481 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
      541 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      601 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      661 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
      721 tcaccatagg cgcgccaggg ccgggattct cctccacgtc accgcatgtt agaagacttc
      781 ctctgccctc tgcgatcgca tcagaaggga agacaagttt ttttcttagt gtgaaagtag
      841 gctgaggcat ctgtttcccc atgtttttct tgtcatcctt gttgcttact tcatagttca
      901 cttcagtcag gatggtgaga atatcatcgc ctcgagggca tcgctctctc aggctctggc
      961 aaagtgactg gatgtaccag gttccctctg cagggtttcg gtaggaaaca cagttattca
     1021 cagtggccat ccc
//
