LOCUS       sequence-165195-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti virus delivery of AsCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY108 (lenti-AsCpf1)
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(141..389)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(199..218)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(369..389)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        438..649
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      657..666
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             669..689
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
       61 gggacagcag agatccagtt tggttaattt aattaaaaaa gagacgatat atcgtctcca
      121 tctacaagag tagaaattac ggtgtttcgt cctttccaca agatatataa agccaagaaa
      181 tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa
      241 actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt
      301 gtgtttacag tcaaattaat tccaattatc tctctaacag ccttgtatcg tatatgcaaa
      361 tatgaaggaa tcatgggaaa taggccctcc tcgagaggtc ttgaaaggag tgggaattgg
      421 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      481 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      541 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      601 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg accggtgcca
      661 ccatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccacacagt
      721 tcgagggctt taccaacctg tatcaggtga gcaagacact gcggtttgag ctgatcccac
      781 agggcaagac cctgaagcac atccaggagc agggcttcat cgaggaggac aaggcccgca
      841 atgatcacta caaggagctg aagcccatca tcgatcggat ctacaagacc tatgccgacc
      901 agtgcctgca gctggtgcag ctggattggg agaacctgag cgccgccatc gactcctata
      961 gaaaggagaa aaccgaggag acaaggaacg ccctgatcga ggagcaggcc acatatcgca
     1021 atgc
//
