LOCUS       sequence-165196-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti virus delivery of AsCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY108 (lenti-AsCpf1)
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(472..528)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(538..618)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(625..672)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      944..953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag
       61 acccttgccc tggtggtcgg gcgagactcc gacggtggcc aggaaccacg cgggctcctt
      121 gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct
      181 caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg
      241 cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag
      301 cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg
      361 atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc
      421 cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcggtccagg
      481 attctcttcg acatctccgg cttgtttcag cagagagaag tttgttgcgc cggatccggc
      541 atagtcgggg acatcatatg ggtatgcata atcaggcacg tcgtagggat aagcgtaatc
      601 tggaacatcg tatgggtagg atcccttttt cttttttgcc tggccggcct ttttcgtggc
      661 cgccggcctt ttgttgcgca gctcctggat gtaggccagc cagtcctgat tggagatgcc
      721 gttctgcagc ttcagatcct tgctctcctt caggtgattc agcagcagct ggcccttcag
      781 ggcgatgtgg taggcgccat tggcatcggc gtccatgggc cactctgggt tctgaaaccg
      841 ggagtcgaag cacacgccat tcagatcgcg cacggggctg ttgatatagt cctcgcctgt
      901 ggcggcattg gagttccgca tctgcagcac gctgcggatc agggccacca tggtgtcgat
      961 ggcgtgagaa tcgtcattct ccagcagctt tggcaggatg ttggagccat ccctgaacac
     1021 ga
//
