LOCUS       sequence-165197-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti virus delivery of LbCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY109 (lenti-LbCpf1)
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(135..383)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(193..212)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(363..383)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        434..645
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      653..662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
       61 agagatccag tttggttaat ttaattaaaa aaagagacga tatatcgtct ccatctacac
      121 ttagtagaaa ttacggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa
      181 tactttcaag ttacggtaag catatgatag tccattttaa aacataattt taaaactgca
      241 aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt
      301 acagtcaaat taattccaat tatctctcta acagccttgt atcgtatatg caaatatgaa
      361 ggaatcatgg gaaataggcc ctcttctcga gaggtcttga aaggagtggg aattggctcc
      421 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      481 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      541 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      601 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtccg gtgccaccat
      661 ggccccaaag aa
//
