LOCUS       sequence-165201-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huAsCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY026
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..80)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcgtaatctg gaacatcgta tgggtaggat ccctttttct tttttgcctg gccggccttt
       61 ttcgtggccg ccggcctttt gttgcgcagc tcctggatgt aggccagcca gtcctgattg
      121 gagatgccgt tctgcagctt cagatccttg ctctccttca ggtgattcag cagcagctgg
      181 cccttcaggg cgatgtggta ggcgccattg gcatcggcgt ccatgggcca ctctgggttc
      241 tgaaaccggg agtcgaagca cacgccattc agatcgcgca cggggctgtt gatatagtcc
      301 tcgcctgtgg cggcattgga gttccgcatc tgcagcacgc tgcggatcag ggccaccatg
      361 gtgtcgatgg cgtgagaatc gtcattctcc agcagctttg gcaggatgtt ggagccatcc
      421 ctgaacacga tgcccttctc ctccagcagg gcgatcagct cgttggcagg atacaggtcc
      481 cggtatctgc cggtgaatct gtgattctcg atcactggca cgattctctt gccggcgatg
      541 aaaggggtgc ccttggcgtc aaactgtgtc tcgttcttct cgaacacgat atcccatgca
      601 ggcataaagc cgggcaggcc cctctggaag gacagatttc tgttcatctt aaagtgcagg
      661 atgaagtcgc cggttttcac gtcgtagtgc agaaagtcga agccctccag gaagtgcttg
      721 cggctctcgt gattcttgat ggttttccac acgaaggggt ccacgaagcc ggtcagggga
      781 tcgatcttag atgtatatgg ggcaggcacg taaaacagga agccagactg ggtgcccatc
      841 ttggcaaagg aggtgaactg gtctgtcagc tggtatgggt tcagcacgcc tcccacttt
//
