LOCUS       sequence-165204-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huLbCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY027
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..143)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(150..197)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattct
       61 taggcatagt cggggacatc atatgggtat gcataatcag gcacgtcgta gggataagcg
      121 taatctggaa catcgtatgg gtaggatccc tttttctttt ttgcctggcc ggcctttttc
      181 gtggccgccg gccttttgtg cttcacgctg gtctgggcgt actccagcca ctccttgtta
      241 gagatggcga tcttcacctt atccagcttc tcgtcctcgg ccttcttgaa ctggccgatg
      301 gcccacagca cctttctggc gatgttatag gcgccattgg cgtcggcgtt ctttggcagg
      361 atggcattct cctgggcctc atagttccgg ctatcgtaga agatgccgtc ggagttcttc
      421 acagggctga tcagaaaatc cacgtcggtg cggcctgtga tgctgttccg catctgcagc
      481 atcaggctca tcagggccat aaagctagag tagaaggcct tgtcggactg ctcgcacagc
      541 agggctctga tatcgccctg ctgataattg atgccgtact tgttgaacag ctccttatag
      601 gcgctggtca ggcacacctc ctcccagtcg aacacgttgt tcttcttagg attccggaag
      661 attctgatcc ggttgccgta ggagtacagc ttccacttct tgatgtaatc ggcgtctgtg
      721 cgagagaagt tcttatagtc cagggcaaac tcgaacagat cctcctcggg cacgtacatg
      781 atcctgtcaa aggagctgat gaacttcttg gaatcggcga tgctggtata cttggttttc
      841 agcaggttca caaagccggt agatggatcg atcttggatg tcagccaggc agggatgtaa
      901 aagatgaagc cgttctgggt agacatggac ttaaagctct cgaacttatt ggtgatctga
      961 tagcccttca gggcgccgcc tgttgc
//
