LOCUS       sequence-165207-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huAsCpf1-T2A-GFP and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY094
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..83)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(99..146)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      418..427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat
       61 gttagcagac ttcctctgcc ctctccactg ccggatccct ttttcttttt tgcctggccg
      121 gcctttttcg tggccgccgg ccttttgttg cgcagctcct ggatgtaggc cagccagtcc
      181 tgattggaga tgccgttctg cagcttcaga tccttgctct ccttcaggtg attcagcagc
      241 agctggccct tcagggcgat gtggtaggcg ccattggcat cggcgtccat gggccactct
      301 gggttctgaa accgggagtc gaagcacacg ccattcagat cgcgcacggg gctgttgata
      361 tagtcctcgc ctgtggcggc attggagttc cgcatctgca gcacgctgcg gatcagggcc
      421 accatggtgt cgatggcgtg agaatcgtca ttctccagca gctttggcag gatgttggag
      481 ccatccctga acacgatgcc cttctcctcc agcagggcga tcagctcgtt ggcaggatac
      541 aggtcccggt atctgccggt gaatctgtga ttctcgatca ctggcacgat tctcttgccg
      601 gcgatgaaag gggtgccctt ggcgtcaaac tgtgtctcgt tcttctcgaa cacgatatcc
      661 catgcaggca taaagccggg caggcccctc tggaaggaca gatttctgtt catcttaaag
      721 tgcaggatga agtcgccggt tttcacgtcg tagtgcagaa agtcgaagcc ctccaggaag
      781 tgcttgcggc tctcgtgatt cttgatggtt ttccacacga aggggtccac gaagccggtc
      841 aggggatcga tcttagatgt atatggggca ggcacgtaaa acaggaagcc agactgggtg
      901 cccatcttgg caaaggaggt gaactggtct gt
//
