LOCUS       sequence-165221-         536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-MBP- NleG6-3 EHEC in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub69-NleG6-3 EHEC
FEATURES             Location/Qualifiers
     source          1..536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..60
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 aataacaata acaacaacct cgggatcgat acgaccctgg aagttctgtt ccaggggccc
       61 ccgcggatgg agagaagggc tgtggctctg gaaaggcaat taaatggagg tgtcgatttt
      121 ttaaggagtg ttaataacta ttttcagagt gtcatggcag aacacagaga aaataaaaca
      181 agtaataaaa tattaatgga aaaaataaat tcctgtgtat ttggaacgga ttctaatcac
      241 ttttcttgcc cggagtcatt tttgacatgc ccgataacgc tggacacacc tgcgaatgga
      301 gtgttcatga gaaactcaca aggtgctgag atatgctctc tatatgataa ggacacgtta
      361 gtgcaacttg ttgaaactgg tggagctcat cctctgagtc gagaacctat aacagaatca
      421 atgattatga gaaaagacga atgtcacttt gattcaaaaa aagaatcctt tgttgcaagt
      481 gatgcttaaa ctagtttaag catcacttgc aacaaagagt gacagagcca cagccc
//
