LOCUS       sequence-165225-        5037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SMURF2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SMURF2_WT
FEATURES             Location/Qualifiers
     source          1..5037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             110..901
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      994..1582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1483..1502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1736..1753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1912..1939)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2031..2117)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2166..2188
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2180..2197
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2181..2197
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4676..4695)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4677..4695)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4700..4716)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4700..4716)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
       61 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
      121 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      181 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      241 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      301 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      361 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      421 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      481 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      541 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      601 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      661 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      721 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      781 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
      841 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
      901 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
      961 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1021 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1081 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1141 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1201 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1261 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1321 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1381 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1441 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1501 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1561 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1621 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     1681 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     1741 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     1801 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     1861 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
     1921 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
     1981 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
     2041 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
     2101 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
     2161 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat
     2221 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
     2281 tataatgcca actttgtaca aaaaggttgg caccatgtct aaccccggag gccggaggaa
     2341 cgggcccgtc aagctgcgcc tgacagtact ctgtgcaaaa aacctggtga aaaaggattt
     2401 tttccgactt cctgatccat ttgctaaggt ggtggttgat ggatctgggc aatgccattc
     2461 tacagatact gtgaagaata cgcttgatcc aaagtggaat cagcattatg acctgtatat
     2521 tggaaagtct gattcagtta cgatcagtgt atggaatcac aagaagatcc ataagaaaca
     2581 aggtgctgga tttctcggtt gtgttcgtct tctttccaat gccatcaacc gcctcaaaga
     2641 cactggttat cagaggttgg atttatgcaa actcgggcca aatgacaatg atacagttag
     2701 aggacagata gtagtaagtc ttcagtccag agaccgaata ggcacaggag gacaagttgt
     2761 ggactgcagt cgtttatttg ataacgattt accagacggc tgggaagaaa ggagaaccgc
     2821 ctctggaaga atccagtatc taaaccatat aacaagaact acgcaatggg agcgcccaac
     2881 acgaccggca tccgaatatt ctagccctgg cagacctctt agctgctttg ttgatgagaa
     2941 cactccaatt agtggaacaa atggtgcaac atgtggacag tcttcagatc ccaggctggc
     3001 agagaggaga gtcaggtcac aacgacatag aaattacatg agcagaacac atttacatac
     3061 tcctccagac ctaccagaag gctatgaaca gaggacaacg caacaaggcc aggtgtattt
     3121 cttacataca cagactggtg tgagcacatg gcatgatcca agagtgccca gggatcttag
     3181 caacatcaat tgtgaagagc ttggtccatt gcctcctgga tgggagatcc gtaatacggc
     3241 aacaggcaga gtttatttcg ttgaccataa caacagaaca acacaattta cagatcctcg
     3301 gctgtctgct aacttgcatt tagttttaaa tcggcagaac caattgaaag accaacagca
     3361 acagcaagtg gtatcgttat gtcctgatga cacagaatgc ctgacagtcc caaggtacaa
     3421 gcgagacctg gttcagaaac taaaaatttt gcggcaagaa ctttcccaac aacagcctca
     3481 ggcaggtcat tgccgcattg aggtttccag ggaagagatt tttgaggaat catatcgaca
     3541 ggtcatgaaa atgagaccaa aagatctctg gaagcgatta atgataaaat ttcgtggaga
     3601 agaaggcctt gactatggag gcgttgccag ggaatggttg tatctcttgt cacatgaaat
     3661 gttgaatcca tactatggcc tcttccagta ttcaagagat gatatttata cattgcagat
     3721 caatcctgat tctgcagtta atccggaaca tttatcctat ttccactttg ttggacgaat
     3781 aatgggaatg gctgtgtttc atggacatta tattgatggt ggtttcacat tgccttttta
     3841 taagcaattg cttgggaagt caattacctt ggatgacatg gagttagtag atccggatct
     3901 tcacaacagt ttagtgtgga tacttgagaa tgatattaca ggtgttttgg accatacctt
     3961 ctgtgttgaa cataatgcat atggtgaaat tattcagcat gaacttaaac caaatggcaa
     4021 aagtatccct gttaatgaag aaaataaaaa agaatatgtc aggctctatg tgaactggag
     4081 atttttacga ggcattgagg ctcaattctt ggctctgcag aaaggattta atgaagtaat
     4141 tccacaacat ctgctgaaga catttgatga gaaggagtta gagctcatta tttgtggact
     4201 tggaaagata gatgttaatg actggaaggt aaacacccgg ttaaaacact gtacaccaga
     4261 cagcaacatt gtcaaatggt tctggaaagc tgtggagttt tttgatgaag agcgacgagc
     4321 aagattgctt cagtttgtga caggatcctc tcgagtgcct ctgcagggct tcaaagcatt
     4381 gcaaggtgct gcaggcccga gactctttac catacaccag attgatgcct gcactaacaa
     4441 cctgccgaaa gcccacactt gcttcaatcg aatagacatt ccaccctatg aaagctatga
     4501 aaagctatat gaaaagctgc taacagccat tgaagaaaca tgtggatttg ctgtggaatt
     4561 gccaactttc tcgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     4621 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     4681 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     4741 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     4801 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     4861 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     4921 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     4981 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcc
//
