LOCUS       sequence-165226-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used for the transformation of rickettsia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, 
            Felsheim RF, Kurtti TJ, Munderloh UG
  TITLE     Development of shuttle vectors for transformation of diverse 
            Rickettsia species.
  JOURNAL   PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub
            2011 Dec 21.
  PUBMED    22216299
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29511. doi"; pages: " 
            10.1371/journal.pone.0029511. Epub 2011 Dec 21"
COMMENT     Sequence Label: pRAM18dRGA[MCS]
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     164..186
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     178..195
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     179..195
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     202..221
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        202..220
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
       61 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      121 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      181 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcggtacc
      241 taaggacata gtcgctagca agcggccgca tttaaatccg cggcctaggt cccgggtacc
      301 ctcagcatac atctttttaa gtcgggcatt ctccgattct aatgccttta gcttggtaat
      361 catcggtaaa tccatacctc cataccttga tctctatata gacataattt ggctgtcact
      421 atgctttgat atagtcattt tacagaatct ccttttttgt tattagaaaa ttctactctt
      481 cttgtcgatt ttgttttagg gaggattacc ttctcttatg tttttgatcc tgtgttttca
      541 cttactttga ctataatttt ttaactaata tccatactcc actctcttaa cccctgcact
      601 agagccaagc ctctgtataa catgagagta attattacgc acccaatccg caataaatct
      661 tgctggcatt gtaaacttta gtgtagaagt atcattgcac tccttcacta ctgctttttc
      721 aaaccaagct gtatacatag cttctcctaa ctcttcaatt aatccttgtc tgatatgatg
      781 ccatgctgta tttgcttcga tcgtatctaa tcccccactt ttcgctaaaa tacctgcatg
      841 tgtaccatca gacacaacat cagatgttat aattttagga tatagcatta ccttttcttc
      901 atctctccgc
//
