LOCUS       sequence-165231-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used for the transformation of rickettsia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, 
            Felsheim RF, Kurtti TJ, Munderloh UG
  TITLE     Development of shuttle vectors for transformation of diverse 
            Rickettsia species.
  JOURNAL   PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub
            2011 Dec 21.
  PUBMED    22216299
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29511. doi"; pages: " 
            10.1371/journal.pone.0029511. Epub 2011 Dec 21"
COMMENT     Sequence Label: pRAM18dSFA[MCS]
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     129..151
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     143..160
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     144..160
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     167..186
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        167..185
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
       61 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
      121 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
      181 atagggcgaa ttcgagctcg gtacctaagg acatagtcgc tagcaagcgg ccgcatttaa
      241 atccgcggcc taggtcccgg gtaccctcag catacatctt tttaagtcgg gcattctccg
      301 attctaatgc ctttagcttg gtaatcatcg gtaaatccat acctccatac cttgatctct
      361 atatagacat aatttggctg tcactatgct ttgatatagt cattttacag aatctccttt
      421 tttgttatta gaaaattcta ctcttcttgt cgattttgtt ttagggagga ttaccttctc
      481 ttatgttttt gatcctgtgt tttcacttac tttgactata attttttaac taatatccat
      541 actccactct cttaacccct gcactagagc caagcctctg tataacatga gagtaattat
      601 tacgcaccca atccgcaata aatcttgctg gcattgtaaa ctttagtgta gaagtatcat
      661 tgcactcctt cactactgct ttttcaaacc aagctgtata catagcttct cctaactctt
      721 caattaatcc ttgtctgata tgatgccatg ctgtatttgc ttc
//
