LOCUS       sequence-165236-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains Golden Gate cassette to express up to four gRNAs and dsRED.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV GG hUbC-dsRED
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      159..168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(345..365)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     563..582
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     757..780
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
       61 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
      121 ggctgcaggt cgactctaga ggatccccgg gtaccggtgc caccatggat agcactgaga
      181 acgtcatcaa gcccttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      241 agttcgagat cgagggcgag ggcgagggca agccctacga gggcacccag accgccaagc
      301 tgcaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc
      361 agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt
      421 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      481 ccgtgaccca ggactcctcc ctgcaggacg gcaccttcat ctaccacgtg aagttcatcg
      541 gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gactctgggc tgggagccct
      601 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggcgctga
      661 agctgaaggg cggcggccac tacctggtgg agttcaagtc aatctacatg gccaagaagc
      721 ccgtgaagct gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg
      781 aggactacac cgtggtggag cagtacgagc gcgccgaggc ccgc
//
