LOCUS       sequence-165237-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains Golden Gate cassette to express up to four gRNAs and dsRED.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV GG hUbC-dsRED
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    65..653
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(118..138)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(536..547)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(656..672)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(657..673)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gtacgagcgc gccgaggccc gccaccacct gttccagtag gaattcgata tcaagcttat
       61 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
      121 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
      181 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
      241 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
      301 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
      361 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
      421 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
      481 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
      541 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
      601 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
      661 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
      721 caatgctgat tgtgcctggc taga
//
