LOCUS       sequence-165250-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector, Firefly Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR-LUC (w158-1)
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(43..142)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(161..184)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(192..219)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(351..394)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatggt ggagcctgct tttttgtaca
       61 aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca
      121 gtcaaaataa aatcattatt tggggcccga gcttaagtaa ctaactaaca ggaagagttt
      181 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
      241 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
      301 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
      361 aggctcagtc ggaagactgg gcctttcgtt ttatttgatg cctggcagtt ccctactctc
      421 gcttagtagt tagacgtccc cgagatccat gctagcggta atacggttat ccacagaatc
      481 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      541 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      601 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taa
//
