LOCUS       sequence-165271-        4938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DVL3, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DVL3_WT
FEATURES             Location/Qualifiers
     source          1..4938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     505..524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     758..775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(934..961)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1053..1139)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1188..1210
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1202..1219
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1203..1219
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1235..1334
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1910..1921
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(3483..3582)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3599..3618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3600..3618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3623..3639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3623..3639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4070..4861
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
       61 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      841 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg
      901 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
      961 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
     1021 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
     1081 gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga
     1141 tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg
     1201 ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat
     1261 agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta
     1321 caaaaaagtt ggcatgggcg agaccaagat catctaccac ttggatgggc aggagacgcc
     1381 gtaccttgtg aagctgcccc tgcccgccga gcgcgtcacc ttggcggact ttaagggcgt
     1441 tttgcagcga cccagctata agttcttctt caagtctatg gacgacgatt tcggagtggt
     1501 gaaggaggag atctcggatg acaatgccaa gctaccatgc ttcaatggcc gggtggtgtc
     1561 ctggctggtg tcagctgagg gctcacaccc agacccagcc cccttctgtg ctgataaccc
     1621 atcggagctg ccaccaccta tggagcgcac gggaggcatc ggggactccc gacccccatc
     1681 cttccaccct catgctggtg ggggcagcca ggagaacctg gacaatgaca cagagacgga
     1741 ctctttggtg tctgcccagc gagagcggcc acgccggagg gatggcccag agcatgcaac
     1801 ccggctaaat ggaactgcga agggggaacg gcggcgagaa ccagggggtt atgatagctc
     1861 atccaccctt atgagcagtg agctggagac caccagcttc tttgactcag atgaggatga
     1921 ctccaccagc aggttcagca gctccacaga acagagcagt gcctcacgcc tgatgagaag
     1981 acacaagcgg cggcggcgga agcagaaggt ttctcggatt gagcggtcct cgtccttcag
     2041 cagcatcacg gactccacca tgtcactcaa catcatcacg gtcactctca acatggaaaa
     2101 atataacttc ttgggcatct ccattgtggg ccaaagcaac gagcgtggtg acggcggcat
     2161 ctacattggc tctatcatga agggtggggc cgtggctgct gatggacgca tcgagccagg
     2221 agatatgttg ttacaggtaa acgagatcaa ctttgagaac atgagtaatg acgatgcagt
     2281 ccgggtactg cgggagattg tgcacaaacc ggggcccatc accctgactg tagccaagtg
     2341 ctgggaccca agtccacgtg gttgcttcac attgcccagg agcgagccca tccggcccat
     2401 tgaccctgcg gcctgggtct cccacactgc agccatgacc ggcaccttcc ctgcatacgg
     2461 catgagcccc tccctgagca ccatcacctc caccagctcc tccatcacca gttccatccc
     2521 tgacacagag cgcctagacg acttccactt gtccatccac agtgacatgg ctgccatcgt
     2581 aaaagccatg gcctcccctg aatcagggtt ggaggtccgt gaccgcatgt tgctcaagat
     2641 taccatccct aatgctttca tcggctcaga tgtggtggac tggctgtacc acaatgtgga
     2701 aggcttcacg gaccggaggg aggcccgcaa gtatgccagc aacctgctga aagctggctt
     2761 catccgccat accgtcaaca agatcacctt ctccgagcag tgctactaca tcttcggtga
     2821 cctctgcggc aacatggcca acctgtctct ccacgatcac gatggctcca gtggcgcctc
     2881 tgaccaggac acactggccc ctttgccgca cccgggggcc gccccttggc ccatggcttt
     2941 cccgtaccag tacccgccac ccccgcaccc atacaacccg cacccgggct tcccggagct
     3001 gggctacagc tacggcgggg gcagcgccag cagtcagcac agcgaaggca gtcggagcag
     3061 tggctccaac cgtagcggca gcgatcggag gaaggagaag gacccgaagg ccggggactc
     3121 caagtccggg ggcagcggca gcgaatcgga ccacaccaca cgcagcagcc tgcgggggcc
     3181 gcgggagcgg gcgcccagcg agcgctcagg gccggcggcc agcgagcaca gccaccgcag
     3241 ccaccattcc ctggccagca gccttcgcag ccaccacaca cacccgagct acggtcctcc
     3301 cggagtgccc cctctctacg gcccccccat gctgatgatg cccccgccgc ccgcggccat
     3361 ggggccccca ggagcccctc cgggccgcga cctggcctca gtgcccccgg aactgaccgc
     3421 cagcagacag tccttccgca tggccatggg aaaccccagt gagttctttg tggatgtgat
     3481 gtgcccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     3541 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     3601 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     3661 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     3721 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     3781 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     3841 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     3901 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     3961 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     4021 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     4081 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     4141 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     4201 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     4261 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     4321 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     4381 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     4441 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     4501 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     4561 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     4621 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     4681 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     4741 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     4801 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     4861 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     4921 gtcagacccc gtagaaaa
//
