LOCUS       sequence-165288-        4526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAP3K7 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP3K7_WT
FEATURES             Location/Qualifiers
     source          1..4526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1007..1018)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1926..2025)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2042..2061)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2043..2061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2066..2082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2066..2082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2513..3304
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3397..3985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3886..3905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4139..4156
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4315..4342)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4434..4520)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gtctacagcc tctgccgcct cctcctcctc ctcgtcttcg gccggtgaga
      241 tgatcgaagc cccttcccag gtcctcaact ttgaagagat cgactacaag gagatcgagg
      301 tggaagaggt tgttggaaga ggagcctttg gagttgtttg caaagctaag tggagagcaa
      361 aagatgttgc tattaaacaa atagaaagtg aatctgagag gaaagcgttt attgtagagc
      421 ttcggcagtt atcccgtgtg aaccatccta atattgtaaa gctttatgga gcctgcttga
      481 atccagtgtg tcttgtgatg gaatatgctg aagggggctc tttatataat gtgctgcatg
      541 gtgctgaacc attgccatat tatactgctg cccacgcaat gagttggtgt ttacagtgtt
      601 cccaaggagt ggcttatctt cacagcatgc aacccaaagc gctaattcac agggacctga
      661 aaccaccaaa cttactgctg gttgcagggg ggacagttct aaaaatttgt gattttggta
      721 cagcctgtga cattcagaca cacatgacca ataacaaggg gagtgctgct tggatggcac
      781 ctgaagtttt tgaaggtagt aattacagtg aaaaatgtga cgtcttcagc tggggtatta
      841 ttctttggga agtgataacg cgtcggaaac cctttgatga gattggtggc ccagctttcc
      901 gaatcatgtg ggctgttcat aatggtactc gaccaccact gataaaaaat ttacctaagc
      961 ccattgagag cctgatgact cgttgttggt ctaaagatcc ttcccagcgc ccttcaatgg
     1021 aggaaattgt gaaaataatg actcacttga tgcggtactt tccaggagca gatgagccat
     1081 tacagtatcc ttgtcagtat tcagatgaag gacagagcaa ctctgccacc agtacaggct
     1141 cattcatgga cattgcttct acaaatacga gtaacaaaag tgacactaat atggagcaag
     1201 ttcctgccac aaatgatact attaagcgct tagaatcaaa attgttgaaa aatcaggcaa
     1261 agcaacagag tgaatctgga cgtttaagct tgggagcctc ccgtgggagc agtgtggaga
     1321 gcttgccccc aacctctgag ggcaagagga tgagtgctga catgtctgaa atagaagcta
     1381 ggatcgccgc aaccacaggc aacggacagc caagacgtag atccatccaa gacttgactg
     1441 taactggaac agaacctggt caggtgagca gtaggtcatc cagtcccagt gtcagaatga
     1501 ttactacctc aggaccaacc tcagaaaagc caactcgaag tcatccatgg acccctgatg
     1561 attccacaga taccaatgga tcagataact ccatcccaat ggcttatctt acactggatc
     1621 accaactaca gcctctagca ccgtgcccaa actccaaaga atctatggca gtgtttgaac
     1681 agcattgtaa aatggcacaa gaatatatga aagttcaaac agaaattgca ttgttattac
     1741 agagaaagca agaactagtt gcagaactgg accaggatga aaaggaccag caaaatacat
     1801 ctcgcctggt acaggaacat aaaaagcttt tagatgaaaa caaaagcctt tctacttact
     1861 accagcaatg caaaaaacaa ctagaggtca tcagaagtca gcagcagaaa cgacaaggca
     1921 cttcatccca actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     1981 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     2041 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     2101 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     2161 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
     2221 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     2281 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     2341 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     2401 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     2461 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
     2521 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     2581 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     2641 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     2701 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     2761 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     2821 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     2881 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     2941 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     3001 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     3061 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     3121 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     3181 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     3241 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     3301 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3541 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4021 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4081 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4141 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4201 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4261 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     4321 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     4381 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     4441 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     4501 tgagcctttc gttttatttg atgcct
//
