LOCUS       sequence-165290-        5631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAP3K14 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP3K14_WT
FEATURES             Location/Qualifiers
     source          1..5631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3031..3130)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3147..3166)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3148..3166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3171..3187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3171..3187)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3618..4409
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4502..5090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4991..5010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5244..5261
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5420..5447)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5539..5625)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggcagtgatg gaaatggcct gcccaggtgc ccctggctca gcagtggggc
      241 agcagaagga actccccaaa gccaaggaga agacgccgcc actggggaag aaacagagct
      301 ccgtctacaa gcttgaggcc gtggagaaga gccctgtgtt ctgcggaaag tgggagatcc
      361 tgaatgacgt gattaccaag ggcacagcca aggaaggctc cgaggcaggg ccagctgcca
      421 tctctatcat cgcccaggct gagtgtgaga atagccaaga gttcagcccc accttttcag
      481 aacgcatttt catcgctggg tccaaacagt acagccagtc cgagagtctt gatcagatcc
      541 ccaacaatgt ggcccatgct acagagggca aaatggcccg tgtgtgttgg aagggaaagc
      601 gtcgcagcaa agcccggaag aaacggaaga agaagagctc aaagtccctg gctcatgcag
      661 gagtggcctt ggccaaaccc ctccccagga cccctgagca ggagagctgc accatcccag
      721 tgcaggagga tgagtctcca ctcggcgccc catatgttag aaacaccccg cagttcacca
      781 agcctctgaa ggaaccaggc cttgggcaac tctgttttaa gcagcttggc gagggcctac
      841 ggccggctct gcctcgatca gaactccaca aactgatcag ccccttgcaa tgtctgaacc
      901 acgtgtggaa actgcaccac ccccaggacg gaggccccct gcccctgccc acgcacccct
      961 tcccctatag cagactgcct catcccttcc cattccaccc tctccagccc tggaaacctc
     1021 accctctgga gtccttcctg ggcaaactgg cctgtgtaga cagccagaaa cccttgcctg
     1081 acccacacct gagcaaactg gcctgtgtag acagtccaaa gcccctgcct ggcccacacc
     1141 tggagcccag ctgcctgtct cgtggtgccc atgagaagtt ttctgtggag gaatacctag
     1201 tgcatgctct gcaaggcagc gtgagctcag gccaggccca cagcccgacc agcctggcca
     1261 agacctgggc agcaaggggc tccagatccc gggagcccag ccccaaaact gaggacaacg
     1321 agggtgtcct gctcactgag aaactcaagc cagtggatta tgagtaccga gaagaagtcc
     1381 actgggccac gcaccagctc cgcctgggca gaggctcctt cggagaggtg cacaggatgg
     1441 aggacaagca gactggcttc cagtgcgctg tcaaaaaggt gcggctggaa gtatttcggg
     1501 cagaggagct gatggcatgt gcaggattga cctcacccag aattgtccct ttgtatggag
     1561 ctgtgagaga agggccttgg gtcaacatct tcatggagct gctggaaggt ggctccctgg
     1621 gccagctggt caaggagcag ggctgtctcc cagaggaccg ggccctgtac tacctgggcc
     1681 aggccctgga gggtctggaa tacctccact cacgaaggat tctgcatggg gacgtcaaag
     1741 ctgacaacgt gctcctgtcc agcgatggga gccacgcagc cctctgtgac tttggccatg
     1801 ctgtgtgtct tcaacctgat ggcctgggaa agtccttgct cacaggggac tacatccctg
     1861 gcacagagac ccacatggct ccggaggtgg tgctgggcag gagctgcgac gccaaggtgg
     1921 atgtctggag cagctgctgt atgatgctgc acatgctcaa cggctgccac ccctggactc
     1981 agttcttccg agggccgctc tgcctcaaga ttgccagcga gcctccgcct gtgagggaga
     2041 tcccaccctc ctgcgcccct ctcacagccc aggccatcca agaggggctg aggaaagagc
     2101 ccatccaccg cgtgtctgca gcggagctgg gagggaaggt gaaccgggca ctacagcaag
     2161 tgggaggtct gaagagccct tggaggggag aatataaaga accaagacat ccaccgccaa
     2221 atcaagccaa ttaccaccag accctccatg cccagccgag agagctttcg ccaagggccc
     2281 cagggccccg gccagctgag gagacaacag gcagagcccc taagctccag cctcctctcc
     2341 caccagagcc cccagagcca aacaagtctc ctcccttgac tttgagcaag gaggagtctg
     2401 ggatgtggga acccttacct ctgtcctccc tggagccagc ccctgccaga aaccccagct
     2461 caccagagcg gaaagcaacc gtcccggagc aggaactgca gcagctggaa atagaattat
     2521 tcctcaacag cctgtcccag ccattttctc tggaggagca ggagcaaatt ctctcgtgcc
     2581 tcagcatcga cagcctctcc ctgtcggatg acagtgagaa gaacccatca aaggcctctc
     2641 aaagctcgcg ggacaccctg agctcaggcg tacactcctg gagcagccag gccgaggctc
     2701 gaagctccag ctggaacatg gtgctggccc gggggcggcc caccgacacc ccaagctatt
     2761 tcaatggtgt gaaagtccaa atacagtctc ttaatggtga acacctgcac atccgggagt
     2821 tccaccgggt caaagtggga gacatcgcca ctggcatcag cagccagatc ccagctgcag
     2881 ccttcagctt ggtgaccaaa gacgggcagc ctgttcgcta cgacatggag gtgccagact
     2941 cgggcatcga cctgcagtgc acactggccc ctgatggcag cttcgcctgg agctggaggg
     3001 tcaagcatgg ccagctggag aacaggccct acccaacttt cttgtacaaa gttggcatta
     3061 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     3121 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     3181 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     3241 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     3301 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     3361 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     3421 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     3481 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     3541 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     3601 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     3661 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     3721 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     3781 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     3841 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     3901 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     3961 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     4021 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     4081 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     4141 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     4201 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     4261 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     4321 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     4381 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     4441 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4501 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4561 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4621 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4681 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4741 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4801 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4861 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4921 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4981 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5041 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5101 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5161 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5221 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5281 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5341 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     5401 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     5461 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     5521 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     5581 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc t
//
