LOCUS       sequence-165292-        9103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for transmembrane protein dimerization..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Armstrong CR, Senes A
  TITLE     Screening for transmembrane association in divisome proteins using 
            TOXGREEN, a high-throughput variant of the TOXCAT assay.
  JOURNAL   Biochim Biophys Acta. 2016 Jul 21. pii: S0005-2736(16)30251-6. doi: 
            10.1016/j.bbamem.2016.07.008.
  PUBMED    27453198
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2016 Jul 21. pii: S0005-2736(16)30251-6. doi: 
            10.1016/j.bbamem.2016.07.008."
COMMENT     Sequence Label: pccGFPKAN
FEATURES             Location/Qualifiers
     source          1..9103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..36)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     78..94
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     79..95
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1245..1264)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1256..2071
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1256..2071
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1329..1348)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             2703..3800
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      3657..3666
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     3774..3797
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     promoter        3865..3969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3970..4038,4039..4830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4188..4207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5001..5589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5490..5509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5743..5760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5775..5915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5841..5860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6001..6023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6017..6208)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(6790..6817)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6909..6995)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(7288..7315)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(7407..7493)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7931..8644)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      complement(9006..9092)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgttcg aggtcgacgg tatcgataag cttgatgggg atcgcgtttc
      121 tttattagtg gttgcagtct cgctcataat cgctccgttt acttctgttt caaacaattg
      181 atccattgag actcaatgga attaccttga tgtgcaagtg agatatggac aaaaaatgta
      241 aattcaaggt caaaactcat aaaaacactg ttttttgatc gagattggat tattctaagt
      301 ctgcattttt atcaaagaag ataaaaaaac cagtaaagtc tgagtgttgg gacagggaga
      361 tactgggaca ttagatgttc ggattaggac acaactcaaa agagatttcg atgagtcata
      421 ttggtactaa attcattctt gctgaaaaat ttaccttcga tcccctaagc aatactctga
      481 ttgacaaaga agatagtgaa gagatcattc gattaggcag caacgaaagc cgaattcttt
      541 ggctgctggc ccaacgtcca aacgaggtaa tttctcgcaa tgatttgcat gactttgttt
      601 ggcgagagca aggttttgaa gtcgatgatt ccagcttaac ccaagccatt tcgactctgc
      661 gcaaaatgct caaagattcg acaaagtccc cacaatacgt caaaacggtt ccgaagcgcg
      721 gttaccaatt gatcgcccga gtggaaacgg ttgaagaaga gatggctcgc gaaaacgaag
      781 ctgctcatga catctctcag ccagaatctg tcaatgaata cgcagaatca agcagtgtgc
      841 cttcatcagc cactgtagtg aacacaccgc agccagccaa tgtcgtggcg aataaatcgg
      901 ctccaaactt ggggaatcga gctagcgctg aggtctgcct cgtgaagaag gtgttgctga
      961 ctcataccag gcctgaatcg ccccatcatc cagccagaaa gtgagggagc cacggttgat
     1021 gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg ccacggaacg
     1081 gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag ttcgatttat
     1141 tcaacaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1201 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     1261 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
     1321 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1381 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1441 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1501 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1561 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1621 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     1681 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1741 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1801 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     1861 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     1921 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     1981 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2041 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2101 gcagagcatt acgctgactt gacgggacgg cggctttgtt gaataaatcg aacttttgct
     2161 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
     2221 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc
     2281 actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca
     2341 cgaggcagac ctcagcgctc aaagatgcag gggtaaaagc taaccgcatc tttaccgaca
     2401 aggcatccgg cagttcaaca gatcgggaag ggctggattt gctgaggatg aaggtggagg
     2461 aaggtgatgt cattctggtg aagaagctcg accgtcttgg ccgcgacacc gccgacatga
     2521 tccaactgat aaaagagttt gatgctcagg gtgtagcggt tcggtttatt gacgacggga
     2581 tcagtaccga cggtgatatg gggcaaatgg tggtcaccat cctgtcggct gtggcacagg
     2641 ctgaacgccg gaggatcctg atcaacccaa gccaatccag ctttaaaccc ctaacggata
     2701 tcaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc
     2761 tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc
     2821 cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta
     2881 tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca
     2941 ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca
     3001 acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag
     3061 atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga
     3121 aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc
     3181 tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag
     3241 acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta
     3301 aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag
     3361 gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag
     3421 tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg
     3481 gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggcg aaagagttcc
     3541 tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg
     3601 gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca
     3661 ccatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct
     3721 ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag
     3781 ccctgaaaga cgcgcagact aattcgagct cggtacccgg ccggggatct taatctagca
     3841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3961 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4021 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4081 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4141 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4201 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4261 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4321 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4381 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4441 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4501 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4801 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4861 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4921 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5041 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5101 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5161 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5221 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5281 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5341 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     5401 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5461 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5521 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5581 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5641 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5701 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5761 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     5821 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     5881 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     5941 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6001 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     6061 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     6121 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     6181 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     6241 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     6301 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     6361 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     6421 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     6481 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     6541 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     6601 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     6661 tcatgcgcac ccgtggccag gacccaacgc tgcccgactg ctttcctgat gcaaaaacga
     6721 ggctagttta ccgtatctgt ggggggatgg cttgtagata tgacgacagg aagagtttgt
     6781 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt
     6841 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct
     6901 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     6961 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
     7021 atggggagac cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg
     7081 gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct
     7141 gcgttctgat ttaatctgta tcaggctgaa aatcttctct catccgccaa aacagctgct
     7201 ttcctgatgc aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg
     7261 acgacaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
     7321 atttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     7381 gcaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     7441 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
     7501 gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac
     7561 ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaattctg
     7621 ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa tcttctctca
     7681 tccgccaaaa cagccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
     7741 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
     7801 attcttggag tggtgaatcc gttagcgagg tgccgcctcg acgaatttct gccattcatc
     7861 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7921 taaaaactca tttgtacagt tcatccatac catgcgtgat gcccgctgcg gttacgaact
     7981 ccagcagaac catgtgatcg cgtttctcgt tcggatcttt agacagaacg ctttgcgtgc
     8041 tcagatagtg attgtctggc agcagaacag gaccatcacc gattggagtg ttttgctggt
     8101 agtgatcagc cagctgcacg ctgccatcct ccacgttgtg gcgaatttta aaattcgctt
     8161 taatgccatt tttttgttta tcggcggtga tgtaaacatt gtggctgtta aaattgtatt
     8221 ccagcttatg gcccaggata ttgccgtctt ctttaaagtc aatgcctttc agctcaatgc
     8281 ggtttaccag ggtatcgcct tcaaatttca cttccgcacg cgttttgtac gtgccgtcat
     8341 ccttaaagga aatcgtgcgt tcctgcacat agccttccgg catggcggac ttgaagaagt
     8401 catgctgctt catgtggtcc ggataacgag caaagcactg aacaccataa gtcagcgtcg
     8461 ttaccagagt cggccaaggt accggcagtt taccagtagt acagatgaac ttcagcgtca
     8521 gtttaccatt agttgcgtca ccttcaccct cgccacgcac ggaaaactta tgaccgttga
     8581 catcaccatc cagttccacc agaataggga cgacaccagt gaacagctct tcgcctttgc
     8641 ccattttagc ttccttagct cctgaaaatc tcgtcgaagc tcggcggatt tgtcctactc
     8701 aagcttgctc cctttgttta acagaaaaat aattgatcaa aacaattaaa taagtaaata
     8761 aatttgtatt atttgaaatc aaaaatcaaa aatcaaaaat caaaaatcaa aaatcaaaaa
     8821 tcaaaatata ctatttagtc cttttttatc attttctgtt tttatcggta aaccccgttt
     8881 cacttctaag cttggctgca ggtcgacgga tcgatccccg ggaattccct ggcagtttat
     8941 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa atccgctccc
     9001 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     9061 cagtctttcg actgagcctt tcgttttatt tgatgcctgg gaa
//
