LOCUS       sequence-165294-        4959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BRD4 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BRD4_WT
FEATURES             Location/Qualifiers
     source          1..4959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2359..2458)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2475..2494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2476..2494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2499..2515)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2499..2515)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2946..3737
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3830..4418
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4319..4338
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4572..4589
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4748..4775)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4867..4953)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtctgcg gagagcggcc ctgggacgag attgagaaat ctgccagtaa
      241 tgggggatgg actagaaact tcccaaatgt ctacaacaca ggcccaggcc caaccccagc
      301 cagccaacgc agccagcacc aaccccccgc ccccagagac ctccaaccct aacaagccca
      361 agaggcagac caaccaactg caatacctgc tcagagtggt gctcaagaca ctatggaaac
      421 accagtttgc atggcctttc cagcagcctg tggatgccgt caagctgaac ctccctgatt
      481 actataagat cattaaaacg cctatggata tgggaacaat aaagaagcgc ttggaaaaca
      541 actattactg gaatgctcag gaatgtatcc aggacttcaa cactatgttt acaaattgtt
      601 acatctacaa caagcctgga gatgacatag tcttaatggc agaagctctg gaaaagctct
      661 tcttgcaaaa aataaatgag ctacccacag aagaaaccga gatcatgata gtccaggcaa
      721 aaggaagagg acgtgggagg aaagaaacag ggacagcaaa acctggcgtt tccacggtac
      781 caaacacaac tcaagcatcg actcctccgc agacccagac ccctcagccg aatcctcctc
      841 ctgtgcaggc cacgcctcac cccttccctg ccgtcacccc ggacctcatc gtccagaccc
      901 ctgtcatgac agtggtgcct ccccagccac tgcagacgcc cccgccagtg cccccccagc
      961 cacaaccccc acccgctcca gctccccagc ccgtacagag ccacccaccc atcatcgcgg
     1021 ccaccccaca gcctgtgaag acaaagaagg gagtgaagag gaaagcagac accaccaccc
     1081 ccaccaccat tgaccccatt cacgagccac cctcgctgcc cccggagccc aagaccacca
     1141 agctgggcca gcggcgggag agcagccggc ctgtgaaacc tccaaagaag gacgtgcccg
     1201 actctcagca gcacccagca ccagagaaga gcagcaaggt ctcggagcag ctcaagtgct
     1261 gcagcggcat cctcaaggag atgtttgcca agaagcacgc cgcctacgcc tggcccttct
     1321 acaagcctgt ggacgtggag gcactgggcc tacacgacta ctgtgacatc atcaagcacc
     1381 ccatggacat gagcacaatc aagtctaaac tggaggcccg tgagtaccgt gatgctcagg
     1441 agtttggtgc tgacgtccga ttgatgttct ccaactgcta taagtacaac cctcctgacc
     1501 atgaggtggt ggccatggcc cgcaagctcc aggatgtgtt cgaaatgcgc tttgccaaga
     1561 tgccggacga gcctgaggag ccagtggtgg ccgtgtcctc cccggcagtg ctccctccca
     1621 ccaaggttgt ggccccgccc tcatccagcg acagcagcag cgatagctcc tcggacagtg
     1681 acagttcgac tgatgactct gaggaggagc gagcccagcg gctggctgag ctccaggagc
     1741 agctcaaagc cgtgcacgag cagcttgcag ccctctctca gccccagcag aacaaaccaa
     1801 agaaaaagga gaaagacaag aaggaaaaga aaaaagaaaa gcacaaaagg aaagaggaag
     1861 tggaagagaa taaaaaaagc aaagccaagg aacctcctcc taaaaagacg aagaaaaata
     1921 atagcagcaa cagcaatgtg agcaagaagg agccagcgcc catgaagagc aagccccctc
     1981 ccacgtatga gtcggaggaa gaggacaagt gcaagcctat gtcctatgag gagaagcggc
     2041 agctcagctt ggacatcaac aagctccccg gcgagaagct gggccgcgtg gtgcacatca
     2101 tccagtcacg ggagccctcc ctgaagaatt ccaaccccga cgagattgaa atcgactttg
     2161 agaccctgaa gccgtccaca ctgcgtgagc tggagcgcta tgtcacctcc tgtttgcgga
     2221 agaaaaggaa acctcaagct gagaaagttg atgtgattgc cggctcctcc aagatgaagg
     2281 gcttctcgtc ctcagagtcg gagagctcca gtgagtccag ctcctctgac agcgaagact
     2341 ccgaaacagg tcctgccttg ccaactttct tgtacaaagt tggcattata agaaagcatt
     2401 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     2461 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2521 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2581 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     2641 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2701 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2761 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2821 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2881 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2941 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     3001 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3061 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     3121 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     3181 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     3241 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     3301 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3361 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3421 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3481 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3541 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3601 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3661 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3721 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     3781 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3841 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3901 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3961 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4021 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4081 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4141 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4201 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4261 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4321 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4381 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4441 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4501 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4561 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4621 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4681 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4741 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4801 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4861 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4921 ccagtcttcc gactgagcct ttcgttttat ttgatgcct
//
