LOCUS       sequence-165303-        5574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RB1, part of the Target Accelerator 
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RB1_WT
FEATURES             Location/Qualifiers
     source          1..5574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(943..970)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1062..1148)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1197..1219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1211..1228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1212..1228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1244..1343
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4128..4227)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4244..4263)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4245..4263)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4268..4284)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4268..4284)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4715..5506
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      841 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      901 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
      961 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     1021 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     1081 gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt
     1141 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
     1201 gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt
     1261 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
     1321 aactttgtac aaaaaagttg gcatgccgcc caaaaccccc cgaaaaacgg ccgccaccgc
     1381 cgccgctgcc gccgcggaac ccccggcacc gccgccgccg ccccctcctg aggaggaccc
     1441 agagcaggac agcggcccgg aggacctgcc tctcgtcagg cttgagtttg aagaaacaga
     1501 agaacctgat tttactgcat tatgtcagaa attaaagata ccagatcatg tcagagagag
     1561 agcttggtta acttgggaga aagtttcatc tgtggatgga gtattgggag gttatattca
     1621 aaagaaaaag gaactgtggg gaatctgtat ctttattgca gcagttgacc tagatgagat
     1681 gtcgttcact tttactgagc tacagaaaaa catagaaatc agtgtccata aattctttaa
     1741 cttactaaaa gaaattgata ccagtaccaa agttgataat gctatgtcaa gactgttgaa
     1801 gaagtatgat gtattgtttg cactcttcag caaattggaa aggacatgtg aacttatata
     1861 tttgacacaa cccagcagtt cgatatctac tgaaataaat tctgcattgg tgctaaaagt
     1921 ttcttggatc acatttttat tagctaaagg ggaagtatta caaatggaag atgatctggt
     1981 gatttcattt cagttaatgc tatgtgtcct tgactatttt attaaactct cacctcccat
     2041 gttgctcaaa gaaccatata aaacagctgt tatacccatt aatggttcac ctcgaacacc
     2101 caggcgaggt cagaacagga gtgcacggat agcaaaacaa ctagaaaatg atacaagaat
     2161 tattgaagtt ctctgtaaag aacatgaatg taatatagat gaggtgaaaa atgtttattt
     2221 caaaaatttt atacctttta tgaattctct tggacttgta acatctaatg gacttccaga
     2281 ggttgaaaat ctttctaaac gatacgaaga aatttatctt aaaaataaag atctagatgc
     2341 aagattattt ttggatcatg ataaaactct tcagactgat tctatagaca gttttgaaac
     2401 acagagaaca ccacgaaaaa gtaaccttga tgaagaggtg aatgtaattc ctccacacac
     2461 tccagttagg actgttatga acactatcca acaattaatg atgattttaa attcagcaag
     2521 tgatcaacct tcagaaaatc tgatttccta ttttaacaac tgcacagtga atccaaaaga
     2581 aagtatactg aaaagagtga aggatatagg atacatcttt aaagagaaat ttgctaaagc
     2641 tgtgggacag ggttgtgtcg aaattggatc acagcgatac aaacttggag ttcgcttgta
     2701 ttaccgagta atggaatcca tgcttaaatc agaagaagaa cgattatcca ttcaaaattt
     2761 tagcaaactt ctgaatgaca acatttttca tatgtcttta ttggcgtgcg ctcttgaggt
     2821 tgtaatggcc acatatagca gaagtacatc tcagaatctt gattctggaa cagatttgtc
     2881 tttcccatgg attctgaatg tgcttaattt aaaagccttt gatttttaca aagtgatcga
     2941 aagttttatc aaagcagaag gcaacttgac aagagaaatg ataaaacatt tagaacgatg
     3001 tgaacatcga atcatggaat cccttgcatg gctctcagat tcacctttat ttgatcttat
     3061 taaacaatca aaggaccgag aaggaccaac tgatcacctt gaatctgctt gtcctcttaa
     3121 tcttcctctc cagaataatc acactgcagc agatatgtat ctttctcctg taagatctcc
     3181 aaagaaaaaa ggttcaacta cgcgtgtaaa ttctactgca aatgcagaga cacaagcaac
     3241 ctcagccttc cagacccaga agccattgaa atctacctct ctttcactgt tttataaaaa
     3301 agtgtatcgg ctagcctatc tccggctaaa tacactttgt gaacgccttc tgtctgagca
     3361 cccagaatta gaacatatca tctggaccct tttccagcac accctgcaga atgagtatga
     3421 actcatgaga gacaggcatt tggaccaaat tatgatgtgt tccatgtatg gcatatgcaa
     3481 agtgaagaat atagacctta aattcaaaat cattgtaaca gcatacaagg atcttcctca
     3541 tgctgttcag gagacattca aacgtgtttt gatcaaagaa gaggagtatg attctattat
     3601 agtattctat aactcggtct tcatgcagag actgaaaaca aatattttgc agtatgcttc
     3661 caccaggccc cctaccttgt caccaatacc tcacattcct cgaagccctt acaagtttcc
     3721 tagttcaccc ttacggattc ctggagggaa catctatatt tcacccctga agagtccata
     3781 taaaatttca gaaggtctgc caacaccaac aaaaatgact ccaagatcaa gaatcttagt
     3841 atcaattggt gaatcattcg ggacttctga gaagttccag aaaataaatc agatggtatg
     3901 taacagcgac cgtgtgctca aaagaagtgc tgaaggaagc aaccctccta aaccactgaa
     3961 aaaactacgc tttgatattg aaggatcaga tgaagcagat ggaagtaaac atctcccagg
     4021 agagtccaaa tttcagcaga aactggcaga aatgacttct actcgaacac gaatgcaaaa
     4081 gcagaaaatg aatgatagca tggatacctc aaacaaggaa gagaaatgcc caactttctt
     4141 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     4201 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     4261 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     4321 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     4381 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     4441 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     4501 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     4561 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     4621 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     4681 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     4741 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     4801 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     4861 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     4921 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     4981 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     5041 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     5101 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     5161 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     5221 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     5281 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     5341 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     5401 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     5461 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     5521 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accc
//
