LOCUS       sequence-165311-        4782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SDHA, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SDHA_WT
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     373..392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     626..643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(802..829)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(921..1007)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1056..1078
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1070..1087
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1071..1087
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1103..1202
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3195..3294)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3311..3330)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3312..3330)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3335..3351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3335..3351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3782..4573
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4666..4782,1..472)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
       61 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      601 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      661 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      721 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca
      781 ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga
      841 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg
      901 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
      961 ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt
     1021 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
     1081 ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc
     1141 gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagttgg
     1201 catgtcgggg gtccggggcc tgtcgcggct gctgagcgct cggcgcctgg cgctggccaa
     1261 ggcgtggcca acagtgttgc aaacaggaac ccgaggtttt cacttcactg ttgatgggaa
     1321 caagagggca tctgctaaag tttcagattc catttctgct cagtatccag tagtggatca
     1381 tgaatttgat gcagtggtgg taggcgctgg aggggcaggc ttgcgagctg catttggcct
     1441 ttctgaggca gggtttaata cagcatgtgt taccaagctg tttcctacca ggtcacacac
     1501 tgttgcagca cagggaggaa tcaatgctgc tctggggaac atggaggagg acaactggag
     1561 gtggcatttc tacgacaccg tgaagggctc cgactggctg ggggaccagg atgccatcca
     1621 ctacatgacg gagcaggccc ccgccgccgt ggtcgagcta gaaaattatg gcatgccgtt
     1681 tagcagaact gaagatggga agatttatca gcgtgcattt ggtggacaga gcctcaagtt
     1741 tggaaagggc gggcaggccc atcggtgctg ctgtgtggct gatcggactg gccactcgct
     1801 attgcacacc ttatatggaa ggtctctgcg atatgatacc agctattttg tggagtattt
     1861 tgccttggat ctcctgatgg agaatgggga gtgccgtggt gtcatcgcac tgtgcataga
     1921 ggacgggtcc atccatcgca taagagcaaa gaacactgtt gttgccacag gaggctacgg
     1981 gcgcacctac ttcagctgca cgtctgccca caccagcact ggcgacggca cggccatgat
     2041 caccagggca ggccttcctt gccaggacct agagtttgtt cagttccacc ccacaggcat
     2101 atatggtgct ggttgtctca ttacggaagg atgtcgtgga gagggaggca ttctcattaa
     2161 cagtcaaggc gaaaggttta tggagcgata cgcccctgtc gcgaaggacc tggcgtctag
     2221 agatgtggtg tctcggtcca tgactctgga gatccgagaa ggaagaggct gtggccctga
     2281 gaaagatcac gtctacctgc agctgcacca cctacctcca gagcagctgg ccacgcgcct
     2341 gcctggcatt tcagagacag ccatgatctt cgctggcgtg gacgtcacga aggagccgat
     2401 ccctgtcctc cccaccgtgc attataacat gggcggcatt cccaccaact acaaggggca
     2461 ggtcctgagg cacgtgaatg gccaggatca gattgtgccc ggcctgtacg cctgtgggga
     2521 ggccgcctgt gcctcggtac atggtgccaa ccgcctcggg gcaaactcgc tcttggacct
     2581 ggttgtcttt ggtcgggcat gtgccctgag catcgaagag tcatgcaggc ctggagataa
     2641 agtccctcca attaaaccaa acgctgggga agaatctgtc atgaatcttg acaaattgag
     2701 atttgctgat ggaagcataa gaacatcgga actgcgactc agcatgcaga agtcaatgca
     2761 aaatcatgct gccgtgttcc gtgtgggaag cgtgttgcaa gaaggttgtg ggaaaatcag
     2821 caagctctat ggagacctaa agcacctgaa gacgttcgac cggggaatgg tctggaacac
     2881 ggacctggtg gagaccctgg agctgcagaa cctgatgctg tgtgcgctgc agaccatcta
     2941 cggagcagag gcacggaagg agtcacgggg cgcgcatgcc agggaagact acaaggtgcg
     3001 gattgatgag tacgattact ccaagcccat ccaggggcaa cagaagaagc cctttgagga
     3061 gcactggagg aagcacaccc tgtcctatgt ggacgttggc actgggaagg tcactctgga
     3121 atatagaccc gtgatcgaca aaactttgaa cgaggctgac tgtgccaccg tcccgccagc
     3181 cattcgctcc tactgcccaa ctttcttgta caaagttggc attataagaa agcattgctt
     3241 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     3301 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     3361 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     3421 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
     3481 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     3541 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     3601 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     3661 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     3721 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     3781 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     3841 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     3901 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     3961 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     4021 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     4081 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     4141 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     4201 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     4261 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     4321 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     4381 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     4441 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     4501 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     4561 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
     4621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact gg
//
