LOCUS       sequence-165325-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huLbCpf1-T2A-GFP and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY095
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..81)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(97..144)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcactgg gccaggattc tcctcgacgt caccgcatgt
       61 tagcagactt cctctgccct ctccactgcc ggatcccttt ttcttttttg cctggccggc
      121 ctttttcgtg gccgccggcc ttttgtgctt cacgctggtc tgggcgtact ccagccactc
      181 cttgttagag atggcgatct tcaccttatc cagcttctcg tcctcggcct tcttgaactg
      241 gccgatggcc cacagcacct ttctggcgat gttataggcg ccattggcgt cggcgttctt
      301 tggcaggatg gcattctcct gggcctcata gttccggcta tcgtagaaga tgccgtcgga
      361 gttcttcaca gggctgatca gaaaatccac gtcggtgcgg cctgtgatgc tgttccgcat
      421 ctgcagcatc aggctcatca gggccataaa gctagagtag aaggccttgt cggactgctc
      481 gcacagcagg gctctgatat cgccctgctg ataattgatg ccgtacttgt tgaacagctc
      541 cttataggcg ctggtcaggc acacctcctc ccagtcgaac acgttgttct tcttaggatt
      601 ccggaagatt ctgatccggt tgccgtagga gtacagcttc cacttcttga tgtaatcggc
      661 gtctgtgcga gagaagttct tatagtccag ggcaaactcg aacagatcct cctcgggcac
      721 gtacatgatc ctgtcaaagg agctgatgaa cttcttggaa tcggcgatgc tggtatactt
      781 ggttttcagc aggttcacaa agccggtaga tggatcgatc ttggatgtca gccaggcagg
      841 gatgtaaaag atgaagccgt tctgggtaga catggactta aagctctcga acttattggt
      901 gatctgatag cccttcaggg cgccgcctgt tgcacaagga ttagacttct tgtccaccat
      961 gtagttcagc ttatcgatca gcatcttctc gaacttct
//
