LOCUS       sequence-165329-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huAsCpf1-P2A-puro and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY30
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gccaccatga cacagttcga gggctttacc aacctgtatc aggtgagcaa gacactgcgg
      181 tttgagctga tcccacaggg caagaccctg aagcacatcc aggagcaggg cttcatcgag
      241 gaggacaagg cccgcaatga tcactacaag gagctgaagc ccatcatcga tcggatctac
      301 aagacctatg ccgaccagtg cctgcagctg gtgcagctgg attgggagaa cctgagcgcc
      361 gccatcgact cctatagaaa ggagaaaacc gaggagacaa ggaacgccct gatcgaggag
      421 caggccacat atcgcaatgc catccacgac tacttcatcg gccggacaga caacctgacc
      481 gatgccatca ataagagaca cgccgagatc tacaagggcc tgttcaaggc cgagctgttt
      541 aatggcaagg tgctgaagca gctgggcacc gtgaccacaa ccgagcacga gaacgccctg
      601 ctgcggagct tcgacaagtt tacaacctac ttctccggct tttatgagaa caggaagaac
      661 gtgttcagcg ccgaggatat cagcacagcc atcccacacc gcatcgtgca ggacaacttc
      721 cccaagttta aggagaattg tcacatcttc acacgcctga tcaccgccgt gcccagcctg
      781 cgggagcact ttgagaacgt gaagaaggcc atcggcatct tcgtgagcac ctccatcgag
      841 gaggtgtttt ccttcccttt ttataaccag ctgctgacac agacccagat cgacctgtat
      901 aaccagctgc tgggaggaat ctctcg
//
