LOCUS       sequence-165331-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huAsCpf1-P2A-puro and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY30
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(384..403)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        510..758
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     510..530
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     681..700
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
      361 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
      421 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
      481 acaaggcaag gcttgaccga cttaattaag agggcctatt tcccatgatt ccttcatatt
      541 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
      601 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      661 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      721 tcttggcttt atatatcttg tggaaaggac gaaacaccgt aatttctact cttgtagatg
      781 gagacgatat atcgtctctt tttcaattgc atgaagaatc tgcttagggt taggcgtttt
      841 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
      901 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      961 taacttacgg taaatggccc gcctggctga ccgcccaac
//
