LOCUS       sequence-165332-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Provost E, Rhee J, Leach SD
  TITLE     Viral 2A peptides allow expression of multiple proteins from a 
            single ORF in transgenic zebrafish embryos.
  JOURNAL   Genesis. 2007 Oct . 45(10):625-9.
  PUBMED    17941043
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2007 Oct . 45(10):625-9."
COMMENT     Sequence Label: pEF6V5:eGFP-CAAX-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     276..297
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(421..439)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(446..463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(446..462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(535..553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          579..782
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(602..622)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
       61 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      121 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
      181 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      241 gccctcgcca gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccagggt
      301 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggagagcg aagggcgaat
      361 tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg cccaattcgc
      421 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaagggcaa ttccaccaca
      481 ctggactagt ggatccgagc tcggtaccaa gcttacctag ccagcttggg tctccctata
      541 gtgagtcgta ttagtaccaa gctaattcct cacgacacct gaaatggaag aaaaaaactt
      601 tgaaccactg tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc
      661 aaggagaatt acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg
      721 ggaaactcca tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc
      781 gagaactaat cgaggtgcct ggacggcgcc cggtactc
//
