LOCUS       sequence-165333-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huAsCpf1-P2A-puro and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY30
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(476..532)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(542..589)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      861..870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc
       61 ccagaccctt gccctggtgg tcgggcgaga ctccgacggt ggccaggaac cacgcgggct
      121 ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac
      181 cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct
      241 ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt
      301 cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt
      361 ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta
      421 cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcggtc
      481 caggattctc ttcgacatct ccggcttgtt tcagcagaga gaagtttgtt gcgccggatc
      541 cctttttctt ttttgcctgg ccggcctttt tcgtggccgc cggccttttg ttgcgcagct
      601 cctggatgta ggccagccag tcctgattgg agatgccgtt ctgcagcttc agatccttgc
      661 tctccttcag gtgattcagc agcagctggc ccttcagggc gatgtggtag gcgccattgg
      721 catcggcgtc catgggccac tctgggttct gaaaccggga gtcgaagcac acgccattca
      781 gatcgcgcac ggggctgttg atatagtcct cgcctgtggc ggcattggag ttccgcatct
      841 gcagcacgct gcggatcagg gccaccatgg tgtcgatggc gtgagaatcg tcattctcca
      901 gcagctttgg caggatgttg gagccatccc tgaacacgat gcccttctcc tccagcaggg
      961 cgatcagctc gttggcagga tacaggtccc ggtatctgcc ggtgaatctg tgattctcga
     1021 tcactggcac gattctct
//
