LOCUS       sequence-165334-        4047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing VEGFC, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_VEGFC_WT
FEATURES             Location/Qualifiers
     source          1..4047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     304..323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     557..574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(733..760)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(852..938)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     987..1009
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1001..1018
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1002..1018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1034..1133
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2391..2490)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2507..2526)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2508..2526)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2531..2547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2531..2547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2978..3769
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3862..4047,1..403)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
       61 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      481 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      541 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      601 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      661 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
      721 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      781 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      841 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      901 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      961 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
     1021 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
     1081 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagttg gcatgcactt
     1141 gctgggcttc ttctctgtgg cgtgttctct gctcgccgct gcgctgctcc cgggtcctcg
     1201 cgaggcgccc gccgccgccg ccgccttcga gtccggactc gacctctcgg acgcggagcc
     1261 cgacgcgggc gaggccacgg cttatgcaag caaagatctg gaggagcagt tacggtctgt
     1321 gtccagtgta gatgaactca tgactgtact ctacccagaa tattggaaaa tgtacaagtg
     1381 tcagctaagg aaaggaggct ggcaacataa cagagaacag gccaacctca actcaaggac
     1441 agaagagact ataaaatttg ctgcagcaca ttataataca gagatcttga aaagtattga
     1501 taatgagtgg agaaagactc aatgcatgcc acgggaggtg tgtatagatg tggggaagga
     1561 gtttggagtc gcgacaaaca ccttctttaa acctccatgt gtgtccgtct acagatgtgg
     1621 gggttgctgc aatagtgagg ggctgcagtg catgaacacc agcacgagct acctcagcaa
     1681 gacgttattt gaaattacag tgcctctctc tcaaggcccc aaaccagtaa caatcagttt
     1741 tgccaatcac acttcctgcc gatgcatgtc taaactggat gtttacagac aagttcattc
     1801 cattattaga cgttccctgc cagcaacact accacagtgt caggcagcga acaagacctg
     1861 ccccaccaat tacatgtgga ataatcacat ctgcagatgc ctggctcagg aagattttat
     1921 gttttcctcg gatgctggag atgactcaac agatggattc catgacatct gtggaccaaa
     1981 caaggagctg gatgaagaga cctgtcagtg tgtctgcaga gcggggcttc ggcctgccag
     2041 ctgtggaccc cacaaagaac tagacagaaa ctcatgccag tgtgtctgta aaaacaaact
     2101 cttccccagc caatgtgggg ccaaccgaga atttgatgaa aacacatgcc agtgtgtatg
     2161 taaaagaacc tgccccagaa atcaacccct aaatcctgga aaatgtgcct gtgaatgtac
     2221 agaaagtcca cagaaatgct tgttaaaagg aaagaagttc caccaccaaa catgcagctg
     2281 ttacagacgg ccatgtacga accgccagaa ggcttgtgag ccaggatttt catatagtga
     2341 agaagtgtgt cgttgtgtcc cttcatattg gcaaagacca caaatgagct acccaacttt
     2401 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2461 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2521 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2581 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     2641 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     2701 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     2761 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2821 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     2881 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     2941 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     3001 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     3061 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     3121 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     3181 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     3241 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     3301 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     3361 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     3421 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     3481 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     3541 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     3601 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     3661 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     3721 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     3781 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     3841 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3901 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3961 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4021 gccgtagtta ggccaccact tcaagaa
//
