LOCUS       sequence-165335-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Provost E, Rhee J, Leach SD
  TITLE     Viral 2A peptides allow expression of multiple proteins from a 
            single ORF in transgenic zebrafish embryos.
  JOURNAL   Genesis. 2007 Oct . 45(10):625-9.
  PUBMED    17941043
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2007 Oct . 45(10):625-9."
COMMENT     Sequence Label: pEF6V5:eGFP-CAAX-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..61)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(431..450)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     700..718
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
       61 cttcgaaccg cgggccctct agactcgagc ggccgccact gtgctggata tctgcagaat
      121 tgcccttggc agagcgaaga gcgagaatcg atttacttgt acagctcgtc catgccgccg
      181 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
      241 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
      301 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
      361 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
      421 cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
      481 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
      541 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
      601 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
      661 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
      721 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
      781 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
      841 tcctcctcgc cct
//
