LOCUS       sequence-165342-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huLbCpf1-P2A-puro and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY31
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(384..403)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        510..758
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     510..530
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     681..700
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
      361 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
      421 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
      481 acaaggcaag gcttgaccga cttaattaag agggcctatt tcccatgatt ccttcatatt
      541 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
      601 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      661 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      721 tcttggcttt atatatcttg tggaaaggac gaaacaccgt aatttctact aagtgtagat
      781 ggagacgata tatcgtctct ttttcaattg catgaagaat ctgcttaggg ttaggcgttt
      841 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      901 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      961 ataacttacg gtaaatggcc cgcctggct
//
