LOCUS       sequence-165344-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HA tagged ADAM10 lacking prodomain and metalloprotease
            domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Gschwind A, Hart S, Fischer OM, Ullrich A
  TITLE     TACE cleavage of proamphiregulin regulates GPCR-induced 
            proliferation and motility of cancer cells.
  JOURNAL   EMBO J. 2003 May 15;22(10):2411-21.
  PUBMED    12743035
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2003-05-15"; volume: "22(10)"; pages: "2411-21"
COMMENT     Sequence Label: pcDNA3-Delta(Pro-MP)ADAM10-HA
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgccacca tggtgttgct gagagtgtta attctgctcc tctcctgggc
      121 ggcggggatg ggagaattcg gccaacctat ttgtggaaat ggaatggtag aacaaggtga
      181 agaatgtgat tgtggctata gtgaccagtg taaagatgaa tgctgcttcg atgcaaatca
      241 accagaggga agaaaatgca aactgaaacc tgggaaacag tgcagtccaa gtcaaggtcc
      301 ttgttgtaca gcacagtgtg cattcaagtc aaagtctgag aagtgtcggg atgattcaga
      361 ctgtgcaagg gaaggaatat gtaatggctt cacagctctc tgcccagcat ctgaccctaa
      421 accaaacttc acagactgta ataggcatac acaagtgtgc attaatgggc aatgtgcagg
      481 ttctatctgt gagaaatatg gcttagagga gtgtacgtgt gccagttctg atggcaaaga
      541 tgataaagaa ttatgccatg tatgctgtat gaagaaaatg gacccatcaa cttgtgccag
      601 tacagggtct gtgcagtgga gtaggcactt cagtggtcga accatcaccc tgcaacctgg
      661 atccccttgc aacgatttta gaggttactg tgatgtcttc atgcggtgca gattagtaga
      721 tgctgatggt cctctagcta ggcttaaaaa agcaattttt agtccagagc tctatgaaaa
      781 cattgctgaa tggattgtgg ctcattggtg ggcagtatta cttatggga
//
