LOCUS       sequence-165345-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huLbCpf1-P2A-puro and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY31
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gccaccatga gcaagctgga gaagtttaca aactgctact ccctgtctaa gaccctgagg
      181 ttcaaggcca tccctgtggg caagacccag gagaacatcg acaataagcg gctgctggtg
      241 gaggacgaga agagagccga ggattataag ggcgtgaaga agctgctgga tcgctactat
      301 ctgtctttta tcaacgacgt gctgcacagc atcaagctga agaatctgaa caattacatc
      361 agcctgttcc ggaagaaaac cagaaccgag aaggagaata aggagctgga gaacctggag
      421 atcaatctgc ggaaggagat cgccaaggcc ttcaagggca acgagggcta caagtccctg
      481 tttaagaagg atatcatcga gacaatcctg ccagagttcc tggacgataa ggacgagatc
      541 gccctggtga acagcttcaa tggctttacc acagccttca ccggcttctt tgataacaga
      601 gagaatatgt tttccgagga ggccaagagc acatccatcg ccttcaggtg tatcaacgag
      661 aatctgaccc gctacatctc taatatggac atcttcgaga aggtggacgc catctttgat
      721 aagcacgagg tgcaggagat caaggagaag atcctgaaca gcgactatga tgtggaggat
      781 ttctttgagg gcgagttctt taactttgtg ctgacacagg agggcatcga cgtgtataac
      841 gccatcatcg gcggcttcgt gaccgagagc ggcgagaaga tcaagggcct gaacgagtac
      901 atcaacctgt ataatcagaa aaccaagcag aagctgccta agtttaagcc actgtataag
      961 caggtgctga gcgatcggga gtctctgagc ttctacggc
//
