LOCUS       sequence-165347-        4977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TBK1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TBK1_WT
FEATURES             Location/Qualifiers
     source          1..4977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      29..617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     518..537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     771..788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(947..974)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1066..1152)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1201..1223
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1215..1232
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1216..1232
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1248..1347
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3535..3634)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3651..3670)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3652..3670)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3675..3691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3675..3691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4122..4913
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
       61 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      301 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      361 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      421 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      481 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      541 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      601 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      661 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      721 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      781 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      841 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      901 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
      961 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     1021 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     1081 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     1141 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
     1201 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
     1261 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
     1321 tgccaacttt gtacaaaaaa gttggcatgc agagcacttc taatcatctg tggcttttat
     1381 ctgatatttt aggccaagga gctactgcaa atgtctttcg tggaagacat aagaaaactg
     1441 gtgatttatt tgctatcaaa gtatttaata acataagctt ccttcgtcca gtggatgttc
     1501 aaatgagaga atttgaagtg ttgaaaaaac tcaatcacaa aaatattgtc aaattatttg
     1561 ctattgaaga ggagacaaca acaagacata aagtacttat tatggaattt tgtccatgtg
     1621 ggagtttata cactgtttta gaagaacctt ctaatgccta tggactacca gaatctgaat
     1681 tcttaattgt tttgcgagat gtggtgggtg gaatgaatca tctacgagag aatggtatag
     1741 tgcaccgtga tatcaagcca ggaaatatca tgcgtgttat aggggaagat ggacagtctg
     1801 tgtacaaact cacagatttt ggtgcagcta gagaattaga agatgatgag cagtttgttt
     1861 ctctgtatgg cacagaagaa tatttgcacc ctgatatgta tgagagagca gtgctaagaa
     1921 aagatcatca gaagaaatat ggagcaacag ttgatctttg gagcattggg gtaacatttt
     1981 accatgcagc tactggatca ctgccattta gaccctttga agggcctcgt aggaataaag
     2041 aagtgatgta taaaataatt acaggaaagc cttctggtgc aatatctgga gtacagaaag
     2101 cagaaaatgg accaattgac tggagtggag acatgcctgt ttcttgcagt ctttctcggg
     2161 gtcttcaggt tctacttacc cctgttcttg caaacatcct tgaagcagat caggaaaagt
     2221 gttggggttt tgaccagttt tttgcagaaa ctagtgatat acttcaccga atggtaattc
     2281 atgttttttc gctacaacaa atgacagctc ataagattta tatacatagc tataatactg
     2341 ctactatatt tcatgaactg gtatataaac aaaccaaaat tatttcttca aatcaagaac
     2401 ttatctacga agggcgacgc ttagtcttag aacctggaag gctggcacaa catttcccta
     2461 aaactactga ggaaaaccct atatttgtag taagccggga acctctggat accataggat
     2521 taatatatga aaaaatttcc ctccctaaag tacatccacg ttatgattta gacggggatg
     2581 ctagcatggc taaggcaata acaggggttg tgtgttatgc ctgcagaatt gccagtacct
     2641 tactgcttta tcaggaatta atgcgaaagg ggatacgatg gctgattgaa ttaattaaag
     2701 atgattacaa tgaaactgtt cacaaaaaga cagaagttgt gatcacattg gatttctgta
     2761 tcagaaacat tgaaaaaact gtgaaagtat atgaaaagtt gatgaagatc aacctggaag
     2821 cggcagagtt aggtgaaatt tcagacatac acaccaaatt gttgagactt tccagttctc
     2881 agggaacaat agaaaccagt cttcaggata tcgacagcag attatctcca ggtggatcac
     2941 tggcagacgc atgggcacat caagaaggca ctcatccgaa agacagaaat gtagaaaaac
     3001 tacaagtcct gttaaattgc atgacagaga tttactatca gttcaaaaaa gaccaagcag
     3061 aacgtagatt agcttataat gaagaacaaa tccacaaatt tgataagcaa aaactgtatt
     3121 accatgccac aaaagctatg acgcacttta cagatgaatg tgttaaaaag tatgaggcat
     3181 ttttgaataa gtcagaagaa tggataagaa agatgcttca tcttaggaaa cagttattat
     3241 cgctgactaa tcagtgtttt gatattgaag aagaagtatc aaaatatcaa gaatatacta
     3301 atgagttaca agaaactctg cctcagaaaa tgtttacagc ttccagtgga atcaaacata
     3361 ccatgacccc aatttatcca agttctaaca cattagtaga aatgactctt ggtatgaaga
     3421 aattaaagga agagatggaa ggggtggtta aagaacttgc tgaaaataac cacattttag
     3481 aaaggtttgg ctctttaacc atggatggtg gccttcgcaa cgttgactgt ctttacccaa
     3541 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     3601 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     3661 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     3721 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     3781 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     3841 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     3901 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     3961 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     4021 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     4081 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     4141 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     4201 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     4261 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     4321 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     4381 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     4441 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     4501 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     4561 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     4621 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     4681 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     4741 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     4801 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     4861 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     4921 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcag
//
