LOCUS       sequence-165348-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses huLbCpf1-P2A-puro and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY31
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(476..532)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(542..589)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc
       61 ccagaccctt gccctggtgg tcgggcgaga ctccgacggt ggccaggaac cacgcgggct
      121 ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac
      181 cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct
      241 ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt
      301 cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt
      361 ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta
      421 cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcggtc
      481 caggattctc ttcgacatct ccggcttgtt tcagcagaga gaagtttgtt gcgccggatc
      541 cctttttctt ttttgcctgg ccggcctttt tcgtggccgc cggccttttg tgcttcacgc
      601 tggtctgggc gtactccagc cactccttgt tagagatggc gatcttcacc ttatccagct
      661 tctcgtcctc ggccttcttg aactggccga tggcccacag cacctttctg gcgatgttat
      721 aggcgccatt ggcgtcggcg ttctttggca ggatggcatt ctcctgggcc tcatagttcc
      781 ggctatcgta gaagatgccg tcggagttct tcacagggct gatcagaaaa tccacgtcgg
      841 tgcggcctgt gatgctgttc cgcatctgca gcatcaggct catcagggcc ataaagctag
      901 agtagaaggc cttgtcggac tgctcgcaca gcagggctct gatatcgccc tgctgataat
      961 tgatgccgta cttgttgaac agctccttat aggcgctggt caggcacacc tcctcccagt
     1021 cgaacac
//
