LOCUS       sequence-165350-        4170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing IRAK4, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IRAK4_WT
FEATURES             Location/Qualifiers
     source          1..4170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     331..350
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     584..601
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(760..787)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(879..965)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1014..1036
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1028..1045
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1029..1045
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1061..1160
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2541..2640)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2657..2676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2658..2676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2681..2697)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2681..2697)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3128..3919
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4012..4170,1..430)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
       61 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      361 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      421 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      481 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      541 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      601 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      661 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      721 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
      781 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
      841 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
      901 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
      961 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
     1021 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg
     1081 actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac
     1141 tttgtacaaa aaagttggca tgaacaaacc cataacacca tcaacatatg tgcgctgcct
     1201 caatgttgga ctaattagga agctgtcaga ttttattgat cctcaagaag gatggaagaa
     1261 gttagctgta gctattaaaa aaccatctgg tgatgataga tacaatcagt ttcacataag
     1321 gagatttgaa gcattacttc aaactggaaa aagtcccact tctgaattac tgtttgactg
     1381 gggcaccaca aattgcacag ttggtgatct tgtggatctt ttgatccaaa atgaattttt
     1441 tgctcctgca agtcttttgc tcccagatgc tgttcccaaa actgctaata cactaccttc
     1501 taaagaagct ataacagttc agcaaaaaca gatgcctttc tgtgacaaag acaggacatt
     1561 gatgacacct gtgcagaatc ttgaacaaag ctatatgcca cctgactcct caagtccaga
     1621 aaataaaagt ttagaagtta gtgatacacg ttttcacagt ttttcatttt atgaattgaa
     1681 gaatgtcaca aataactttg atgaacgacc catttctgtt ggtggtaata aaatgggaga
     1741 gggaggattt ggagttgtat ataaaggcta cgtaaataac acaactgtgg cagtgaagaa
     1801 gcttgcagca atggttgaca ttactactga agaactgaaa cagcagtttg atcaagaaat
     1861 aaaagtaatg gcaaagtgtc aacatgaaaa cttagtagaa ctacttggtt tctcaagtga
     1921 tggagatgac ctctgcttag tatatgttta catgcctaat ggttcattgc tagacagact
     1981 ctcttgcttg gatggtactc caccactttc ttggcacatg agatgcaaga ttgctcaggg
     2041 tgcagctaat ggcatcaatt ttctacatga aaatcatcat attcatagag atattaaaag
     2101 tgcaaatatc ttactggatg aagcttttac tgctaaaata tctgactttg gccttgcacg
     2161 ggcttctgag aagtttgccc agacagtcat gactagcaga attgtgggaa caacagctta
     2221 tatggcacca gaagctttgc gtggagaaat aacacccaaa tctgatattt acagctttgg
     2281 tgtggtttta ctagaaataa taactggact tccagctgtg gatgaacacc gtgaacctca
     2341 gttattgcta gatattaaag aagaaattga agatgaagaa aagacaattg aagattatat
     2401 tgataaaaag atgaatgatg ctgattccac ttcagttgaa gctatgtact ctgttgctag
     2461 tcaatgtctg catgaaaaga aaaataagag accagacatt aagaaggttc aacagctgct
     2521 gcaagagatg acagcttctt acccaacttt cttgtacaaa gttggcatta taagaaagca
     2581 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2641 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2701 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2761 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2821 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2881 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2941 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     3001 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     3061 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     3121 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     3181 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     3241 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3301 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3361 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3421 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3481 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3541 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3601 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3661 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3721 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3781 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3841 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3901 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3961 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4021 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4081 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4141 gcagatacca aatactgttc ttctagtgta
//
