LOCUS       sequence-165353-        3612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PDCD1LG2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PDCD1LG2_WT
FEATURES             Location/Qualifiers
     source          1..3612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      35..623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     524..543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     777..794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(953..980)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1072..1158)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1207..1229
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1221..1238
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1222..1238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1254..1353
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2176..2275)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2292..2311)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2293..2311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2316..2332)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2316..2332)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2763..3554
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
       61 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      901 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
      961 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     1021 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     1081 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     1141 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     1201 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     1261 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     1321 ttataatgcc aactttgtac aaaaaagttg gcaccatgat cttcctcctg ctaatgttga
     1381 gcctggaatt gcagcttcac cagatagcag ctttattcac agtgacagtc cctaaggaac
     1441 tgtacataat agagcatggc agcaatgtga ccctggaatg caactttgac actggaagtc
     1501 atgtgaacct tggagcaata acagccagtt tgcaaaaggt ggaaaatgat acatccccac
     1561 accgtgaaag agccactttg ctggaggagc agctgcccct agggaaggcc tcgttccaca
     1621 tacctcaagt ccaagtgagg gacgaaggac agtaccaatg cataatcatc tatggggtcg
     1681 cctgggacta caagtacctg actctgaaag tcaaagcttc ctacaggaaa ataaacactc
     1741 acatcctaaa ggttccagaa acagatgagg tagagctcac ctgccaggct acaggttatc
     1801 ctctggcaga agtatcctgg ccaaacgtca gcgttcctgc caacaccagc cactccagga
     1861 cccctgaagg cctctaccag gtcaccagtg ttctgcgcct aaagccaccc cctggcagaa
     1921 acttcagctg tgtgttctgg aatactcacg tgagggaact tactttggcc agcattgacc
     1981 ttcaaagtca gatggaaccc aggacccatc caacttggct gcttcacatt ttcatcccct
     2041 cctgcatcat tgctttcatt ttcatagcca cagtgatagc cctaagaaaa caactctgtc
     2101 aaaagctgta ttcttcaaaa gacacaacaa aaagacctgt caccacaaca aagagggaag
     2161 tgaacagtgc tatcttgcca actttcttgt acaaagttgg cattataaga aagcattgct
     2221 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     2281 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     2341 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     2401 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     2461 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     2521 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2581 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     2641 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2701 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2761 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2821 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2881 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2941 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     3001 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     3061 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     3121 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     3181 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     3241 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     3301 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     3361 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     3421 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     3481 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     3541 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     3601 cgttccactg ag
//
