LOCUS       sequence-165362-        3846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SETD8, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SETD8_WT
FEATURES             Location/Qualifiers
     source          1..3846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      42..630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     531..550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     784..801
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(960..987)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1079..1165)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1214..1236
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1228..1245
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1229..1245
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1261..1360
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2417..2516)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2533..2552)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2534..2552)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2557..2573)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2557..2573)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3004..3795
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
       61 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      181 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      661 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      721 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
      781 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
      841 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      901 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
      961 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     1021 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     1081 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     1141 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     1201 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     1261 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     1321 tgctttttta taatgccaac tttgtacaaa aaagttggca tggctagagg caggaagatg
     1381 tccaagcccc gcgcggtgga ggcggcggcg gcggcggcgg cggtggcagc gacggccccg
     1441 ggcccggaga tggtggagcg gaggggcccg gggaggcccc gcaccgacgg ggagaacgta
     1501 tttaccgggc agtcaaagat ctattcctac atgagcccga acaaatgctc tggaatgcgt
     1561 ttcccccttc aggaagagaa ctcagttaca catcacgaag tcaaatgcca ggggaaacca
     1621 ttagccggaa tctacaggaa acgagaagag aaaagaaatg ctgggaacgc agtacggagc
     1681 gccatgaagt ccgaggaaca gaagatcaaa gacgccagga aaggtcccct ggtacctttt
     1741 ccaaaccaaa aatctgaagc agcagaacct ccaaaaactc caccctcatc ttgtgattcc
     1801 accaatgcag ccatcgccaa gcaagccctg aaaaagccca tcaagggcaa acaggccccc
     1861 cgaaaaaaag ctcaaggaaa aacgcaacag aatcgcaaac ttacggattt ctaccctgtc
     1921 cgaaggagct ccaggaagag caaagccgag ctgcagtctg aagaaaggaa aagaatagat
     1981 gaattgattg aaagtgggaa ggaagaagga atgaagattg acctcatcga tggcaaaggc
     2041 aggggtgtga ttgccaccaa gcagttctcc cggggtgact ttgtggtgga ataccacggg
     2101 gacctcatcg agatcaccga cgccaagaaa cgggaggctc tgtacgcaca ggacccttcc
     2161 acgggctgct acatgtacta ttttcagtat ctgagcaaaa cctactgcgt ggatgcaact
     2221 agagagacaa atcgcctagg aagactgatc aatcacagca aatgtgggaa ctgccaaacc
     2281 aaactgcacg acatcgacgg cgtacgtcac ctcatcctca tcgcctcccg agacatcgcg
     2341 gctggggagg agctcctgta tgactatggg gaccgcagca aggcttccat tgaagcccac
     2401 ccgtggctga agcattaccc aactttcttg tacaaagttg gcattataag aaagcattgc
     2461 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     2521 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     2581 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc
     2641 ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg
     2701 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt
     2761 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     2821 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     2881 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     2941 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     3001 attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     3061 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     3121 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     3181 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     3241 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     3301 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     3361 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     3421 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     3481 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     3541 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     3601 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     3661 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     3721 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     3781 gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg
     3841 tcgttc
//
